In the present study, a new thiourea derivative bearing benzothiazole ligand, 1-(benzo[d]thiazol-2yl)thiourea (btt) and its ternary metal (Cu(II), Co(II) and Ni(II)) complexes were synthesized. The structural characterization was carried out by micro analysis, IR, 1 H-NMR, EPR, UV-Visible spectral analyses, molar conductance and thermal analysis studies. Spectral studies of complexes revealed that the metal complexes have distorted octahedral geometry. Molecular modelling study was performed to evaluate the recognition of target compounds at the 3MNG binding pocket. The docking results revealed that copper complex selectively binds to the crucial amino acid residues in the active site of 3MNG. The in vitro antioxidant activity of the ligand and its metal complexes was assayed by radical scavenging activity (DPPH, H 2 O 2 and NO) and ferric reducing antioxidant power (FRAP) methods. The ligand showed moderate antioxidant activity whereas the metal complexes exhibited better antioxidant activity than that of the ligand. The results of the four methods proved that the copper complex is the most potent antioxidant among all the tested compounds.

The aim of this study was synthesis of a new hybrid material and its application in solid phase extraction of metal ions from environmental samples. The hybrid material was synthesized by grafting 3-aminopropyl trimethoxy silane onto β-zeolite and functionalizing with 2,6-diacetyl pyridine in a stepwise covalent process. This hybrid material was characterized by FT-IR and XRD studies. The hybrid material was used as sorbent in solid phase extraction of Pb(II), Ni(II), Cu(II) and Cd(II). The method is based on the collection of metal ions onto 2,6-DAP-PA-β-zeolite and the sorbed metal ions were eluted with 10 mL of 1 M HNO 3. The influences of analytical parameters such as pH of the sample solution, volume of sample, type and volume of eluent, flow rate of the sample and eluent that govern the efficiency and throughput of the method were evaluated. The influence effects of matrix ions (common ions other than the analyte that are present in the sample) on the retentions of the metal ions were also examined. The maximum adsorption capacity values for the metal ions onto 2,6-DAP-PA-β-zeolite, as calculated from the Langmuir model, were 112.7, 94.2, 105, and 102.8 mg g −1 , respectively. The relative standard deviation under optimum conditions was lower than 3.10%. The limits of detection were 0.035 for Pb(II), 0.076 for Ni(II), 0.083 for Cu(II) and 0.059 mg L −1 for Cd(II), respectively. The accuracy of the method was estimated by analyzing reference standard materials. The results indicate that the method is efficient for the solid phase extraction of trace levels of Pb(II), Ni(II), Cu(II) and Cd(II) from environmental samples.