A model of risk regulation is proposed to explain how people balance the goal of seeking closeness to a romantic partner against the opposing goal of minimizing the likelihood and pain of rejection. The central premise is that confidence in a partner's positive regard and caring allows people to risk seeking dependence and connectedness. The risk regulation system consists of 3 interconnected "if--then" contingency rules, 1 cognitive, 1 affective, and 1 behavioral. The authors describe how general perceptions of a partner's regard structure the sensitivity of these 3 "if--then" rules in risky relationship situations. The authors then describe the consequences of such situated "if--then" rules for relationship well-being and conclude by integrating other theoretical perspectives and outlining future research directions.

It is proposed that satisfaction is associated with idealistic, rather than realistic, perceptions of one's partner. To provide baselines for assessing relationship illusions, both members of married and dating heterosexual couples were asked to rate themselves and their partners on a variety of interpersonal attributes. Participants also rated the typical and ideal partner on these attributes. Path analyses revealed that individuals' impressions of their partners were more a mirror of their self-images and ideals than a reflection of their partners' self-reported attributes. Overall, intimates saw their partners in a more positive light than their partners saw themselves. Furthermore, these idealized constructions predicted greater satisfaction. Individuals were happier in their relationships when they idealized their partners and their partners idealized them. Taken together, these results suggest that a certain degree of idealization or illusion may be a critical feature of satisfying dating and even marital relationships.In the case of love, realities model themselves enthusiastically on one's desires. . . it is the passion in which violent desire is most completely satisfied. (Beyle Stendhal, De L'Amour) As Stendhal's musings in De L'Amour illustrate, people immersed in the experience of romantic love often appear to bend reality to the will of their hopes and desires. Rather than being constrained by the sometimes-disappointing reality of their partners' actual attributes, individuals may view their partners through the rosy niters provided by images of ideal partners. Within such idealized constructions, intimates may even see virtues in one another's apparent faults. For example, individuals may preserve feelings of confidence in their romantic relationships-in the face of the doubts posed by a partner's failings-by weaving stories that depict such faults in the best possible light (Murray

The structure and dynamics of bacterial communities in the airways of persons with cystic fibrosis (CF) remain largely unknown. We characterized the bacterial communities in 126 sputum samples representing serial collections spanning 8-9 y from six age-matched male CF patients. Sputum DNA was analyzed by bar-coded pyrosequencing of the V3-V5 hypervariable region of the 16S rRNA gene, defining 662 operational taxonomic units (OTUs) from >633,000 sequences. Bacterial community diversity decreased significantly over time in patients with typically progressive lung disease but remained relatively stable in patients with a mild lung disease phenotype. Antibiotic use, rather than patient age or lung function, was the primary driver of decreasing diversity. Interpatient variability in community structure exceeded intrapatient variability in serial samples. Antibiotic treatment was associated with pronounced shifts in community structure, but communities showed both short-and longterm resilience after antibiotic perturbation. There was a positive correlation between OTU occurrence and relative abundance, with a small number of persistent OTUs accounting for the greatest abundance. Significant changes in community structure, diversity, or total bacterial density at the time of pulmonary exacerbation were not observed. Despite decreasing community diversity in patients with progressive disease, total bacterial density remained relatively stable over time. These findings show the critical relationship between airway bacterial community structure, disease stage, and clinical state at the time of sample collection. These features are the key parameters with which to assess the complex ecology of the CF airway. microbial ecology | microbiome | antibiotics

Aspergillus nidulans is an important experimental organism, and it is a model organism for the genus Aspergillus that includes serious pathogens as well as commercially important organisms. Gene targeting by homologous recombination during transformation is possible in A. nidulans, but the frequency of correct gene targeting is variable and often low. We have identified the A. nidulans homolog (nkuA) of the human KU70 gene that is essential for nonhomologous end joining of DNA in double-strand break repair. Deletion of nkuA (nkuAD) greatly reduces the frequency of nonhomologous integration of transforming DNA fragments, leading to dramatically improved gene targeting. We have also developed heterologous markers that are selectable in A. nidulans but do not direct integration at any site in the A. nidulans genome. In combination, nkuAD and the heterologous selectable markers make up a very efficient genetargeting system. In experiments involving scores of genes, 90% or more of the transformants carried a single insertion of the transforming DNA at the correct site. The system works with linear and circular transforming molecules and it works for tagging genes with fluorescent moieties, replacing genes, and replacing promoters. This system is efficient enough to make genomewide gene-targeting projects feasible.

This article reviews the development of the new U.S. lung allocation system that took effect in spring 2005. In 1998, the Health Resources and Services Administration of the U.S. Department of Health and Human Services published the Organ Procurement and Transplantation Network (OPTN) Final Rule. Under the rule, which became effective in 2000, the OPTN had to demonstrate that existing allocation policies met certain conditions or change the policies to meet a range of criteria, including broader geographic sharing of organs, reducing the use of waiting time as an allocation criterion and creating equitable organ allocation systems using objective medical criteria and medical urgency to allocate donor organs for transplant. This mandate resulted in reviews of all organ allocation policies, and led to the creation of the Lung Allocation Subcommittee of the OPTN Thoracic Organ Transplantation Committee. This paper reviews the deliberations of the Subcommittee in identifying priorities for a new lung allocation system, the analyses under-