Several studies are going on to study the effects of transfusing stored RBCs on recipients. Several biochemical changes occur in stored blood, which may have some medical effects on the recipients. The biochemical changes may vary from donar to donar as well as from Blood bank to Blood bank. Also effects of transfused RBCs will vary from recipient to recipient. Lot of clinical trial should be taken before assessing the safety of stored RBCs. OBJECTIVESTo study the various biochemical changes occurring in a stored whole blood. MATERIAL AND METHODSAnalysis of biochemical changes occurring in stored whole blood in 20 voluntary donars was done in a Blood bank of a Tertiary Hospital. RESULTSOf the 6 biochemical parameters studied, only significant changes were seen in potassium and albumin after storage. There was a significant decrease in albumin concentration and steady increase in potassium values. CONCLUSIONCertain biochemical changes do occur in stored whole blood over a duration of storage. As the demand for blood is high due to various medical conditions, the treating consultant should be aware of the biochemical changes seen in stored whole blood.

Aim and objective:The objective of this study was to correlate laboratory tests during the evolution of dengue fever, comparing frequencies between the different clinical forms in order to use test results to predict the severity of the disease. Methods: This is an observational, descriptive and retrospective study of 50 patients with clinical and serological diagnoses of dengue fever. The tests analyzed were blood count, platelet count, and serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) concentrations. Results: Thrombocytopenia and elevated transaminases were observed in patients with classic dengue fever. The main laboratory abnormalities found in dengue hemorrhagic fever were thrombocytopenia, hemoconcentration and elevated transaminases, similar to severe dengue with the exception of hemoconcentration. Most laboratory abnormalities started on the 3rd day but were more evident on the 5th day with restoration of values by the 11 th day; this was more prominent in under 15-year-olds and with the more severe clinical forms. Conclusion: These results are relevant in assessing the disease because they can be used as markers for more severe forms and can help by enabling the adaptation of the therapeutic conduct to the needs of individual patients.

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BACKGROUNDTrichomonas vaginalis (TV) is a parasitic protozoan that infects the genitourinary tract and produces the most common curable sexually transmitted disease (STD) in sexually active women.The aim was to study the prevalence of Trichomonas vaginalis infection in patients. MATERIALS AND METHODSThis was a 6-month prospective study of all the Pap smear specimens received in the department of pathology. The smears were stained with Pap stain and H & E stain. After microscopy, if the trophozoite forms of T. vaginalis are seen the diagnosis is confirmed.The clinical details were taken from the case reports and requisition slips. RESULTSDuring this study, total 1650 Pap smears were received in the department of pathology out of which 149 patients were positive for the trophozoites making the prevalence rate in this study as 9.03% The 30-to 39-year-old age group had a significantly higher prevalence of infection (36.23 %; P < 0.05) than the 20-to 29-year-old (27.51 %) and 40-to 49-year-old age groups (20.13 %; P > 0.05). The lowest rates of infection were observed in those <20 years of age (6.71 %) and >50 years of age (9.39 %; P > 0.05). CONCLUSIONThe prevalence of TV was more in sexually active age group and its diagnosis is important as it can cause adverse effects in Antenatal care (ANC) period.