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This article brings together what until now have been separate fields of nineteenth-century history: the development of experimental physiology, the growth of mechanized industry, and the city, where their threads intertwined. The main argument is that the laboratory in the city employed the same technological and organizational approaches to modernize that the city used to industrialize. To bring the adoption of technology into focus, the article discusses laboratory research as it developed after the introduction of small-scale power engines. With its machines, the industrialized city provided not only the key metaphor of the nineteenth-century life sciences but also a key technology that shifted experimental practices in animal research from a kind of preindustrial craft to a more mechanized production of knowledge. With its "factory-laboratories," the late-nineteenth-century city became the birthplace for the first living, data-producing hybird---part animal and part machine.