The aim of the research was to test the effect of foliar-applied solutions of ascorbic acid (AsA), glycine betaine (GB), salicylic acid (SA) and water as a second control on the yield and seed germination of the soybean (Glycine hispida (Moench) Maxim.) cultivar 'Sava' as well as their influence on the content of N, P, K and Ca in soybean seeds, and to estimate the intensity of lipid peroxidation and free proline content in soybean seedlings. The plants were treated with the solutions twice. The 1 st spray treatment was done at growth stage 6: flowering (main shoot), when first flowers opened (sporadically in population), and the 2 nd spray treatment was done at growth stage 7: development of fruits and seeds, when the first pod had reached final length of 15-20 mm. Foliar application of the solutions had a significant impact on seed yield and seed germination in both years of the experiment. In a favourable year 2016, treatments with ascorbic acid, glycine betaine and salicylic acid solution significantly increased seed yield by 6.21-9.67%. However, in an unfavourable year 2017, the effect was significantly greater with the application of water (yield increase 6.48%), while the application of glycine betaine solution even reduced the seed yield. In a favourable year 2016, treatments with ascorbic acid and salicylic acid solution significantly increased seed germination by 7% and 8%, respectively. In an unfavourable year 2017, also only ascorbic acid and salicylic acid significantly increased seed germination by 11% and 9%, respectively. However, when using cold test, when the seed was exposed to a low temperature of 10°C for 7 days and then placed under optimal seed germination conditions for 4 days, ascorbic acid and glycine betaine solution reduced seed germination in both experimental years. Therefore, further research is needed on the methods designed to increase seed germination of soybean, both in favourable and adverse conditions in Serbia.

Background: High and stable production requires quality seed. Seed quality is the basis of efficient crop production and farmers need such seeds for optimum yield production. Therefore, various procedures are applied in seed production technology that aim to improve not only the germination of seeds but also the speed of its germination. These methods can reduce of seed aging and the effects of different agroecological factors. Methods: For research the impact of priming used seed aged seven months (fresh seeds) and seed was nineteen months old (aged seed). The seed was treated with following solutions: potassium nitrate (1%), ascorbic acid (100mgl-1) and potassium chloride (1%) for 6 hours and then germinated at 25°C in 8 days. Result: The analysis showed that seed aging resulted in a decrease in its germination energy and germination. Moreover, time required for germination is prolonged, the lipid peroxidation intensity and content of free proline are increased and the amount of vitamin C is reduced. The results showed that the effect of priming is dependable on variety selection, seed age and treatments. Therefore, we can conclude that there is no universal use of one only primer, as it may not be suitable for each particular cultivar and can ultimately lead to a decrease in the germination energy and germination.

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Experiment was conducted in 2014, 2015 and 2016 at the Institute of Field and Vegetable Crops, Novi Sad, Serbia, to study the effects of water deficit treatments during a soybean reproductive stage. Two water deficit treatments were conducted T1 (from beginning of blossom to seed maturation) and T2 (from beginning of seed filling to seed maturation period) and control (no water deficit). The results showed that water deficit during seed formation has a significant effect on germination energy, germination precentage and abnormal seedling. Obtained results are of great importance for farmers, because under favorable conditions, a large number of abnormal seedling has the ability to sprout, reach the reproductive stage and participate in yield formation.

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