Sheep grazing was investigated as an alternative to traditional management of meadows in the Krkonoše Mts. Until the second World War these meadows were mown in mid-summer and grazed by cattle for the rest of the season. Subsequent abandonment of the meadows has resulted in decreasing species richness. Degradation phases of the former communities have been replacing the original species-rich vegetation. Significant changes were apparent six years after the introduction of sheep grazing. In grazed plots the proportion of dominant herbs (Polygonum bistorta and Hypericum maculatum) decreased and grasses (Deschampsia cespitosa, Festuca rubra, Agrostis capillaris, Anthoxanthum alpinum) increased. The increase in grasses was positively correlated with an increase in several herbs. The proportion of some herbs increased despite being selectively grazed (Adenostyles alliariae, Melandrium rubrum, Veratrum lobelianum). Any losses caused by grazing of mature plants were probably compensated by successful seedling establishment. Cessation of grazing resulted in significant changes in vegetation within three years. The cover of nitrophilous tall herbs and grasses (e.g. Rumex alpestris, Holcus mollis, Deschampsia cespitosa, Geranium sylvaticum) increased in the abandoned plots. In the plots grazed for nine years cover of species-rich mountain meadow species increased (e.g. fine-leaved grasses, Campanula bohemica, Potentilla aurea, Viola lutea, Silene vulgaris).The main conservation risk is the expansion of a competitive species with low palatability, Deschampsia cespitosa. This species can be suppressed by a combination of grazing and mowing. In order for grazing to be effective, the number of sheep should be proportional to meadow production. This may be difficult to maintain as production is variable and is impossible to predict at the beginning of a growing season. A large part of the biomass may thus remain intact in some years. Negative effects of grazing may be, at least partly, eliminated by a combination of cutting and grazing.

Vertical differentiation in root placement is one of the potential mechanisms of plant niche differentiation. It can be due to the remarkable plasticity of roots in response to nutrients and neighbours, but most data on it come from pot or garden experiments. The roles of vertical differentiation and of plasticity in it in the field are thus not well known. We examined species‐specific root vertical distribution in a montane grassland using quantitative real‐time PCR. We asked whether individual species differ in their rooting depths, whether such differences are associated with above‐ground functional traits (such as height or specific leaf area), and whether they respond to the presence of a competitor. This response was assessed by comparison of species‐specific vertical profiles between control plots and plots where the dominant species, Festuca rubra, had been removed. Vertical profiles of individual species varied considerably, from species with most root biomass concentrated in the uppermost (<2 cm) soil layer, through species with uniform vertical distribution, to a species with roots predominantly below 8 cm (Nardus stricta). Species at the fast end of the plant economy spectrum were more likely to place their roots in the uppermost layers. Grassland species, thus, exploit different parts of the below‐ground resources in spite of their short stature, minor differences in height above‐ground and shallow soil. While below‐ground and above‐ground biomasses of most species were higher in the removal plots, species rooting patterns did not change in response to the removal. The interspecific differences in vertical profiles were thus due to species' innate differences, not to plastic responses to the presence of the dominant species. Synthesis. The findings imply that vertical root differentiation in the field is strong and can contribute to niche differentiation. However, the role of root plasticity in natural systems may be considerably weaker than in artificial systems with few species and strong nutrient gradients. This absence of the plastic response in the field is likely to be due to a fairly homogeneous distribution of nutrients in the soil and to the predominantly symmetric nature of below‐ground competition.

Festuca rubra , a clonal grass of mountain grasslands, possesses a considerable variability in traits related to spatial spreading (rhizome production, length and branching; tussock architecture). Since these traits highly influence the success of the species in a spatially heterogeneous system of grasslands, a combined field and growth chamber approach was adopted to determine the within‐population variation in these parameters. Clones were sampled in a mountain grassland (The Krkonoše Mts., Czech Republic); the environment (mean neighbour density) of individual clones varied highly. Before the clones were collected, shoot demography and tussock architecture within these clones were recorded in the field for four seasons. Their clone identity was determined using DNA RAPD. Vegetatively propagated plants from these clones were cultivated in a common garden experiment to demonstrate variation in tussock growth and architecture. Their response to change in red/far red light ratio was determined in the growth chamber. Highly significant variation among clones was found in almost all parameters. In the common garden, the clones differed in tussock growth (mean tillering rate), architecture (mean shoot angle, mean tussock density) and proportion of flowering shoots. In the growth chamber, both the aboveground parameters and parameters of the rhizome system were strongly affected by red/far red ratio; among‐clone variation was also almost always significant. The genotype × environment interaction was significant for tillering rate and rhizome architecture. The structure of the rhizome system (which is the major component of clonal spread in space) is a complex result of several components whose inter‐clone variations differ: (i) genetically determined mean rhizome system size, (ii) overall plasticity in rhizome system size (with no significant genetic variation in plasticity), and (iii) genetically determined plasticity in rhizome architecture. Because of the variation in plasticity in rhizome architecture, some c lones seemed to possess the ability to exploit a favourable habitat patch by producing short branches when there; whereas the remaining clones appeared to possess only a simple escape mechanism from unfavourable patches. Environmental variation in the light levels in the studied grassland is fine grained; horizontal growth rates of F. rubra are sufficient to make genets experience different patches in their lifetime. The high variation in both genotype means and plasticities is likely to be due to selection early in genet life in an environment which is heterogeneous at a fine scale.

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