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SUMMARYElectrophoretic patterns of esterases and other soluble proteins from two isolates of Agrobacterium rhizogenes, three of A . tumefaciens, two of Corynebacterium faciens, eight of C. michiganense, three of Pseudomonas phaseolicola, two of Erwinia carotovora, and four of Xanthomonas fragariae were determined. Similar protein patterns were obtained for isolates representing a given bacterial species, with the exception of one isolate of A. tumefaciens which differed markedly from the other two isolates tested. All isolates possessed active esterases. None of the Corynebacterium isolates tested showed activity bands for dehydrogenases. Results demonstrated the applicability of the technique in differentiating between phytobacterial genera and between species of a given genus.

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A rapid, semiquantitative method for immunoelectrophoretic research is described. It combines the great resolving capacity of polyacrylamide gel disc electrophoresis with rapid immunoprecipitation in a thin agar film surrounding the acrylamide gel.