Rheumatoid arthritis (RA) is an autoimmune disease associated with thrombotic complications. To elucidate pathogenic mechanisms, hemostatic disorders in RA were correlated with other laboratory and clinical manifestations. Hemostasis was assessed using relatively new complementary tests, the spatial growth of a plasma clot (Thrombodynamics assay), and contraction of whole blood clots. Platelet functionality was assessed with flow cytometry that quantified the expression of P-selectin and the fibrinogen-binding capacity of platelets before and after activation with a thrombin receptor-activating peptide. Parameters of fibrin clot growth and the kinetics of contraction of blood clots were significantly altered in patients with RA compared to the control group. In Thrombodynamics measurements, an increase in the clot growth rate, size, and optical density of plasma clots altogether indicated chronic hypercoagulability. The rate and extent of blood clot contraction in patients with RA was significantly reduced and associated with platelet dysfunction revealed by an impaired response to activation. Changes in the parameters of clot growth and contraction correlated with the laboratory signs of systemic inflammation, including hyperfibrinogenemia. These results confirm the pathogenic role of hemostatic disorders in RA and support the validity of fibrin clot growth and the blood clot contraction assay as indicators of a (pro)thrombotic state.

Objective. To investigate the prevalence and clinical manifestations of FA to fish in children in Tomsk region. Patients and methods. In the screening stage the crosssectional study based on screening questionnaires in random samples of children aged 7-10 years (n=13 010) was performed as a part of EuroPrevall project (EuroRrevall, № FP62006TTCTU5 Proposal 045 879). Those, who reported adverse reactions to food in the screening stage were considered as cases (n=652), children without reported reactions were controls (n=636). The casecontrol stage included the completion of a clinical questionnaire, skinprick test to fish (ALK Abellö, Spain), measurement of serum total and specific IgE to fish, componentresolved diagnostics: Cyp c 1, Gad m 1 (ImmunoCAP, ISAC, Phadia, Sweden). Results. The prevalence of verified FA to fish in children 7-10 years in the Tomsk region amounted to 0,32%; in urban children - 0,32%, in rural children - 0,31% (p=0,9). The main manifestations were skin symptoms, oral allergy syndrome, gastrointestinal symptoms. Anaphylactic shock developed in 15% of children with FA to fish. Conclusion. Fish is one of the main triggers of FA in children in Tomsk region, which leads to severe clinical manifestations , including lifethreatening conditions.

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