Accumulating evidence indicates that cancer-initiating cells (CICs) are responsible for cancer initiation, relapse, and metastasis. Colorectal carcinoma (CRC) is typically classified into proximal colon, distal colon, and rectal cancer. The gradual changes in CRC molecular features within the bowel may have considerable implications in colon and rectal CICs. Unfortunately, limited information is available on CICs derived from rectal cancer, although colon CICs have been described. Here we identified rectal CICs (R-CICs) that possess differentiation potential in tumors derived from patients with rectal adenocarcinoma. The R-CICs carried both CD44 and CD54 surface markers, while R-CICs and their immediate progenies carried potential epithelial–mesenchymal transition characteristics. These R-CICs generated tumors similar to their tumor of origin when injected into immunodeficient mice, differentiated into rectal epithelial cells in vitro, and were capable of self-renewal both in vitro and in vivo. More importantly, subpopulations of R-CICs resisted both 5-fluorouracil/calcium folinate/oxaliplatin (FolFox) and cetuximab treatment, which are the most common therapeutic regimens used for patients with advanced or metastatic rectal cancer. Thus, the identification, expansion, and properties of R-CICs provide an ideal cellular model to further investigate tumor progression and determine therapeutic resistance in these patients.

Treatment outcomes of Angle Class II subdivision malocclusions may be compromised because of the uncertainty of the aetiology. Previous studies have reported controversial ideas about the origins, but the existence of a primary contributor still remains unknown. Functional factors have been mentioned as a probable cause, but until now, there have been no supporting data. This study was a cross-sectional investigation of the characteristics of Angle Class II subdivision malocclusion, including dental, skeletal and functional factors, by comparison of the subdivision group and the normal occlusion group. The evaluations of dental and skeletal asymmetries of both groups were carried out by cone-beam computed tomography (CBCT) and analysis of dental casts. The functional deviations were evaluated by cast mounting and measuring. In the subdivision group, the asymmetric position of the glenoid fossa was found to be the most significant skeletal asymmetry. No dentoalveolar asymmetry was found in this group. The most important finding was that, in subdivision malocclusions, functional deviation resulting in pseudoasymmetry occurred in 32.86% of the study participants. This deviation is probably related to the disharmonious arch width between maxillary and mandibular dental arches in the premolar section. The origin of Angle Class II subdivision malocclusion is multifactorial, with dental, skeletal and functional factors included. Functional deviation occurs, probably due to dental arch width disharmony. Asymmetric position of the glenoid fossa may account for most of the skeletal asymmetry.

To provide support for future pharmacology and preclinical studies, we have established a stable nonhuman primate animal model to demonstrate the histopathological changes in the gastrointestinal tract following gamma ray irradiation. In this study, 12 healthy rhesus monkeys were divided into 2 groups (control and radiation groups). Animals in the radiation group were exposed to gamma rays (cobalt 60 source) at a dose level of 6.5 Gy total body irradiation bilaterally (i.e. 3.25 Gy on each side). Control animals were sham exposed using identical procedures. After a 5-day in-life observation period, gastrointestinal tract tissues (esophagus, stomach, duodenum, jejunum, ileum, colon, and rectum) were collected and fixed in 10% neutral-buffered formalin for subsequent hematoxylin and eosin and 5-bromo-2-deoxyuridine (BrdU) immunohistochemistry processing. The results showed that the esophagus was undergoing degeneration without obvious inflammatory changes, while the stomach and duodenum exhibited both degeneration and inflammation. From the jejunum to the rectum, late-stage inflammation with glandular regeneration, as well as a high-level BrdU labeling index, was present.