Peroxidase-labeled Protein A, stable immunoenzyme tracer of high reactivity and comparatively low molecular weight, has been applied in immuno-electron microscopy for detection of bound IgG-type pemphigus and bullous pemphigoid antibodies. Comparing Protein A-peroxidase with peroxidase-labeled immunoglobulins, we obtained similar morphological results in corresponding incubation techniques, but lower nonspecific adsorption of Protein A-peroxidase complexes in tissues. The Protein A-peroxidase molecules showed good tissue penetration abilities. Our rapid one-step incubation procedure led to enhanced preservation of tissue fine structures, without the need of prior tissue fixation. It seems that Protein A-peroxidase is able to replace peroxidase-labeled anti-IgG for immuno-electron microscopical purposes.

Protein A from Staphylococcus aureus, characterized by high affinity binding properties for IgG-type antibodies, was labeled with peroxidase to form a stable immuno-histological tracer molecule of comparatively low molecular weight. It has been used for demonstration of antibodies against tissue antigens, in direct and indirect techniques, and the findings were consistent with those in routine immunofluorescence and in staining with peroxidase-coupled anti-IgG. In comparison, the lowest unspecific tissue adsorption and staining was obtained with Protein A-peroxidase in buffer containing glucose and mannose. The immunohistological preparations were mounted and stored for documentation without apparent loss of staining.

Zusammenfassung. Die seltene erworbene reaktiv perforierende Dermatose (ERPD) wurde bisher vor allem bei Diabetes mellitus und chronischer Niereninsuffizienz beschrieben und gehört wie Hyperkeratosis follicularis et parafollicularis in cutem penetrans (M. Kyrle), perforierende Folliculitis (PF), Elastosis perforans serpiginosa (EPS) und hereditär-infantile reaktiv perforierende Dermatose (HRPD) zur Gruppe der primär perforierenden Dermatosen. Wir berichten kasuistisch über eine ausgeprägte ERPD einer 75-jährigen Patientin bei langjährig bestehender Psoriasis vulgaris. Beschrieben wird die erfolgreiche Therapie mit Allopurinol per os. Die Zugehörigkeit der ERPD zur Gruppe der primär perforierenden Dermatosen wird unterstrichen. Acquired Reactive Perforating Dermatosis in Association with Psoriasis vulgaris-Successful Treatment with Allopurinol-Case Report. Acquired reactive perforating dermatosis (ERPD) has above all been described to be associated with diabetes mellitus and chronic renal failure. This rare disorder belongs to the group of the primary perforating dermatoses like hyperkeratosis follicularis et parafollicularis in cutem penetrans (Kyrle's disease), perforating folliculitis (PF), elastosis perforans serpiginosa (EPS) and hereditary reactive perforating dermatosis of childhood (HRPD). We report on a 75-year old woman with marked ERPD associated with a long-standing Psoriasis vulgaris. Successful treatment with orally administered allopurinol is demonstrated. We stress the fact that ERPD belongs to the group of the primary perforating dermatoses.

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