INTRODUCTION: Canine blood banking in veterinary medicine is an expanding market. Once the demand for blood products increased all over the world, canine blood banks have focused attention on the risk of spreading diseases through blood transfused products. The need to preserve a healthy donor-pool, free of blood-borne infectious diseases, mainly in endemic areas, led to the implementation of appropriate protocols for screening canine blood donors using specific tests. OBJECTIVES: The aim of this study was to evaluate the presence of Anaplasma phagocytophilum/Anaplasmaplatys, Echrlichiacanis/Echrlichiaewingii, Dirofilariaimmitis and Borrelia burgdorferi using the enzyme immunoassay technology (EIA) among the donors of a Romanian canine blood bank, from January 2015 to December 2016. METHODS: Blood samples from 575 donors were collected and 1253 tests were performed with SNAP 4DX Plus® (IDEXX Laboratories, Fremont, CA) to reveal the presence of D. immitis antigens and the antibodies toward A. phagocytophilum and/or A. platys, E. canis and/or E. ewingii and B. burgdorferi. RESULTS: The results of this holistic approach show that all blood samples provided negative results for B. burgdorferi and E.canis/E. ewingii (0/1253), while 0.87% (11/1253) samples provided positive results for A. phagocytophilum/A. platys and 6.94% (87/1253) for D. immitis. CONCLUSION: The next studied topic would be to compare the results provided by the EIA technology with results of real time PCR and qPCR regarding these vector-borne pathogens. UDC Classification: 616.9; DOI: http://dx.

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INTRODUCTION: Seventy-six samples were collected from sixteen canine blood donors at every blood donation performed in accord with the canine donor selection guidelines. Statistical analysis of ELISA OD values revealed no significant variation of anti-rabies antibodies values during repeat donations. Our results support the statement that blood donation does not affect the immune status of donors. OBJECTIVES: The study aimed to assess the immune status against rabies among the canine blood donors in a Romanian canine blood bank METHODS: Serum samples (n=76) were collected from pet owned dogs (n=16) recorded in the blood bank register of a Romanian blood bank were performed with the immunological status of the investigated canine blood donors was evaluated using the indirect ELISA commercial kit PLATELIA RABIES II (BIO-RAD, France) as per the manufacturer's instructions RESULTS: The overall picture of ELISA OD results revealed non-significant variation between and within D1-D6 groups of values (p>0.05, F<Fcrit). CONCLUSION: The next studies topic would be to compare the results provided by the ELISA technology with FAVN.

Background. Malignant tumors of the head and neck have an increasing incidence in the population worldwide, including population of Romania. From all the tumors of the head and neck region oral cavity and pharynx tumors, with extension towards the larynx, have a great impact on the overall survival rates, functional implications and quality of life of the patients. Despite early symptoms, patients have a tendency to present themselves to the specialist in late stages of the disease because of ignorance, fear of diseases or bad advice from unknowing persons. This situation is a great challange for the oncology team considering the poor prognosis of locally advanced cancer. Development of malignant tumors of the oral cavity and pharynx in specific anatomical regions leads to particular ways of approach for each tumor located in different regions of the head and neck. Surgery is the main thod in locally advaced tumors of the oral cavity and pharynx alongside with neck dissection for regional metastasis in lymph nodes. Material and methods. Tumor resection needs to be performed with oncological safe margins of resection which means that a good exposure of the site of the tumor needs to be obtained. In the case of oral cavity and pharynx tumors there are several possibilities of approach. T1 and T2 tumors can be resected by using a transoral approach. T3 tumors have a great local extension thus the need for a better exposure of the site of location through the check. The greater tumors in diameter, T4 tumors, need to be approached by a transmandibular procedure so that the entire tumors can be resected with oncological safe margins of resections. The authors present their experience with the locally advanced malignant tumors of the oral cavity and pharynx in Coltea E.N.T. Clinic. Results. The use of the transmandibular approach in managing cases of locally advanced tumors of the oral cavity and pharynx has some complications that can be avoided if a proper surgical technique is used. Patients operated using the transmandibular technique have a good quality of life despite some complications associated with radiotherapy performed after surgery.