INTRODUCTION: Maedi-Visna (MV) and Caprine Arthritis Encephalitis (CAE) are specific diseases of small ruminants caused by lentiretroviruses. These diseases are not a public health concern but they are important due to their economic impact. OBJECTIVES: The aim of this study is to analyse the results of the serological screening for MVV and CAEV antibodies in samples collected from small ruminants farms located in 14 Romanian counties and the proximity of Bucharest city. METHODS: The samples were analysed by indirect ELISA, using a commercial diagnosis kit. We investigated 702 serum samples in 160 iELISA tests: 148 pools (2-5 samples/pool) and 12 individual animals. RESULTS: We noticed 27.50% positive samples that confirm the exposure to MVV and CAEV viruses of small ruminants, but the confirmation is requesting investigation using molecular biology tools or other serological techniques. CONCLUSION: A reliable picture over the SRLV infections could underlie a national program for monitoring. UDC Classification: 616.9,

Rabies is a viral zoonosis caused by a virus of the genus Lyssavirus, Rhabdoviridae family, which is responsible for a mortality rate of almost 100%, known as one of the most feared zoonotic diseases worldwide (Sudhi Ranjan Garg, 2014). Although half of the world's population lives in endemic areas, more than 95% of the world's fatal cases of rabies occur in Africa and Asia, which are the continents with the highest risk of human mortality (OIE, 2015).

A biological technique wich is extensivelly used in the antigen detection and measurement is enzime linked immunosorbent assay (ELISA). In veterinary medicine, this technique is ussualy used in surveillance or control programs to establish antigenic prevalence and as a diagnostic tool for antigenic type detection (e.g., RHDV typing). In vaccinology, potency assessment (PA) of the vaccine batches is based more frequently on antibody detection using appropriate methods and less in using antigenic methods. The aim of paper is to assess the reliability of an in-house RHDV-Ag sandwich ELISA (sELISA) for PA. Batches of RHDV vaccine containing VSHI-CN-6 strain were tested with sELISA. Using the same batches, the potency tests were carried out according with European Pharmacopoeia 9.0. Healthy rabbits were vaccinated and the immune response was evaluated. The sELISA positive values (OD>300) were at the dilutions >1/64. The HI values of rabbit sera immunised with the same vaccine batches ranged between 1/128 and 1/2048. The sELISA could be a reliable alternative for the evaluation of the potency test for the RHDV vaccine batches. The sELISA is faster (1-2 days) than HI (>42 days) and is carried in respect with the regulation concerning the use of animals for medical purposes.

INTRODUCTION: Seventy-six samples were collected from sixteen canine blood donors at every blood donation performed in accord with the canine donor selection guidelines. Statistical analysis of ELISA OD values revealed no significant variation of anti-rabies antibodies values during repeat donations. Our results support the statement that blood donation does not affect the immune status of donors. OBJECTIVES: The study aimed to assess the immune status against rabies among the canine blood donors in a Romanian canine blood bank METHODS: Serum samples (n=76) were collected from pet owned dogs (n=16) recorded in the blood bank register of a Romanian blood bank were performed with the immunological status of the investigated canine blood donors was evaluated using the indirect ELISA commercial kit PLATELIA RABIES II (BIO-RAD, France) as per the manufacturer's instructions RESULTS: The overall picture of ELISA OD results revealed non-significant variation between and within D1-D6 groups of values (p>0.05, F<Fcrit). CONCLUSION: The next studies topic would be to compare the results provided by the ELISA technology with FAVN.