Serological Results of MVV and Caev Antibodies in Samples Collected From Sheep and Goat Flocks of Romania

Enache, Dan Alexandru; Bărăităreanu, Stelian; Dan, Marius; Gurău, Maria Rodica; Nuţiu, Camelia; Sarbu, Camelia; Daneş, Doina

doi:10.12955/cbup.v7.1444

Cited by 2 publications

(2 citation statements)

References 32 publications

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“…Overall, the available information on the SRLV situation in Romania is very scarce. There are only a few studies about the prevalence of SRLVs in goats, in which a small number of herds and individual animals were investigated [24][25][26][27][28]. Therefore, the real epidemiological status of SRLVs in Romania is unknown.…”

Section: Discussionmentioning

confidence: 99%

First Molecular Characterization of Small Ruminant Lentiviruses Detected in Romania

Olech,

Hodor,

Toma

et al. 2023

Animals

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Small ruminant lentiviruses (SRLVs) are a group of retroviruses that cause multisystem chronic diseases in goats and sheep and lead to production losses in these animals, negatively affecting animal health and welfare. Although molecular characterization of SRLV field isolates has been performed in many countries, there is currently no information on SRLV genotypes circulating in sheep and goats in Romania. Therefore, the main objective of this study was to conduct a molecular and phylogenetic analysis of SRLVs from Romania and determine the degree of genetic relatedness of the obtained sequences to other known SRLV reference strains. A total of 81 sheep lung tissue samples and 41 sheep lung lymph node samples were tested using nested real-time PCR, and samples positive for real-time PCR were used to amplify an 800 bp gag-pol fragment and an overlapping 625 bp fragment of the gag gene. Pairwise DNA distance and phylogenetic analysis showed that the Romanian SRLV strains were closely related to the A2 and A3 strains based on gag-pol sequences and to the A3 and A17 subtypes based on gag sequences. No recombination events were found. Our results revealed that the Romanian sequences have similar epitope patterns to other existing subtypes, although E/K and R/K mutations in epitope 3 were found only in the Romanian sequences, which may have potential value in serological diagnosis. This study is the first report on the genetic characterization of SRLV strains circulating in Romania and provides new information on SRLV heterogeneity. Further detailed studies should be conducted to better understand the divergence of SRLV Romanian strains.

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Section: Discussionmentioning

confidence: 99%

First Molecular Characterization of Small Ruminant Lentiviruses Detected in Romania

Olech,

Hodor,

Toma

et al. 2023

Animals

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“…Maedi-Visna (MV) disease, which was first isolated in Iceland, is a viral infection caused by lentiviruses that affects sheep in two different forms of respiratory and nervous system (1)(2)(3). MV virus is an exogenous lentivirus, non-oncogenic, and is included in the retroviridae family (4).…”

Section: Introductionmentioning

confidence: 99%

Kars İlindeki Bazı Koyun Sürülerinde Maedi-Visna Enfeksiyonunun Seroprevalansının Araştırılması

Gezer

Akyüz

Gökçe

2021

Dicle Üniversitesi Veteriner Fakültesi Dergisi

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Maedi-Visna (MV) is a viral disease that has no treatment and vaccination in sheep which causes chronic respiratory problems, nervous disorders, and yield losses. The aim of this study was to investigate the seroprevalence of MV infection in sheep in Kars province. A total of 200 sheep blood sera taken from 4 local areas (Ortaköy, Boğatepe, Halefoğlu, Borluk) in Kars center were the material of the study. A Commercial ELISA antibody test was used to investigate MV antibodies. At the end of the analysis, 32 of 200 (16%) animals were positive against Maedi-Visna spesific antibodies. At the end of this study, no statistically significant difference was found between the groups in terms of breed, location and gender. Only the difference between ages was determined. In conclusion, this study shows that MV disease occurs in sheep in Kars province.

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Serological Results of MVV and Caev Antibodies in Samples Collected From Sheep and Goat Flocks of Romania

Cited by 2 publications

References 32 publications

First Molecular Characterization of Small Ruminant Lentiviruses Detected in Romania

First Molecular Characterization of Small Ruminant Lentiviruses Detected in Romania

Kars İlindeki Bazı Koyun Sürülerinde Maedi-Visna Enfeksiyonunun Seroprevalansının Araştırılması

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