Maedi-Visna (MV) is a viral disease that has no treatment and vaccination in sheep which causes chronic respiratory problems, nervous disorders, and yield losses. The aim of this study was to investigate the seroprevalence of MV infection in sheep in Kars province. A total of 200 sheep blood sera taken from 4 local areas (Ortaköy, Boğatepe, Halefoğlu, Borluk) in Kars center were the material of the study. A Commercial ELISA antibody test was used to investigate MV antibodies. At the end of the analysis, 32 of 200 (16%) animals were positive against Maedi-Visna spesific antibodies. At the end of this study, no statistically significant difference was found between the groups in terms of breed, location and gender. Only the difference between ages was determined. In conclusion, this study shows that MV disease occurs in sheep in Kars province.