BackgroundCattle are bred for, amongst other factors, specific traits, including parasite resistance and adaptation to climate. However, the influence and inheritance of mitochondrial DNA (mtDNA) are not usually considered in breeding programmes. In this study, we analysed the mtDNA profiles of cattle from Victoria (VIC), southern Australia, which is a temperate climate, and the Northern Territory (NT), the northern part of Australia, which has a tropical climate, to determine if the mtDNA profiles of these cattle are indicative of breed and phenotype, and whether these profiles are appropriate for their environments.ResultsA phylogenetic tree of the full mtDNA sequences of different breeds of cattle, which were obtained from the NCBI database, showed that the mtDNA profiles of cattle do not always reflect their phenotype as some cattle with Bos taurus phenotypes had Bos indicus mtDNA, whilst some cattle with Bos indicus phenotypes had Bos taurus mtDNA. Using D-loop sequencing, we were able to contrast the phenotypes and mtDNA profiles from different species of cattle from the 2 distinct cattle breeding regions of Australia. We found that 67 of the 121 cattle with Bos indicus phenotypes from NT (55.4%) had Bos taurus mtDNA. In VIC, 92 of the 225 cattle with Bos taurus phenotypes (40.9%) possessed Bos indicus mtDNA. When focusing on oocytes from cattle with the Bos taurus phenotype in VIC, their respective oocytes with Bos indicus mtDNA had significantly lower levels of mtDNA copy number compared with oocytes possessing Bos taurus mtDNA (P < 0.01). However, embryos derived from oocytes with Bos indicus mtDNA had the same ability to develop to the blastocyst stage and the levels of mtDNA copy number in their blastocysts were similar to blastocysts derived from oocytes harbouring Bos taurus mtDNA. Nevertheless, oocytes originating from the Bos indicus phenotype exhibited lower developmental potential due to low mtDNA copy number when compared with oocytes from cattle with a Bos taurus phenotype.ConclusionsThe phenotype of cattle is not always related to their mtDNA profiles. MtDNA profiles should be considered for breeding programmes as they also influence phenotypic traits and reproductive capacity in terms of oocyte quality.Electronic supplementary materialThe online version of this article (doi:10.1186/s12863-017-0523-5) contains supplementary material, which is available to authorized users.

Context Phosphorus (P) deficiency occurs in beef cattle grazing many rangeland regions with low-P soils, including in northern Australia, and may severely reduce cattle productivity in terms of growth, reproductive efficiency and mortality. However, adoption of effective P supplementation by cattle producers in northern Australia is low. This is likely to be due to lack of information and understanding of the profitability of P supplementation where cattle are P-deficient. Aims The profitability of P supplementation was evaluated for two dissimilar regions of northern Australia, namely (1) the Katherine region of the Northern Territory, and (2) the Fitzroy Natural Resource Management (NRM) region of central Queensland. Methods Property-level, regionally relevant herd models were used to determine whole-of-business productivity and profitability over 30 years. The estimated costs and benefits of P supplementation were obtained from collation of experimental data and expert opinion of persons with extensive experience of the industry. The economic consequences of P supplementation at the property level were assessed by comparison of base production without P supplementation with the expected production of P-supplemented herds, and included the implementation phase and changes over time in herd structure. In the Katherine region, it was assumed that the entire cattle herd (breeders and growing cattle) grazed acutely P-deficient land types and the consequences of (1) no P supplementation, or P supplementation during (2) the dry season, or (3) both the wet and dry seasons (i.e. 3 scenarios) were evaluated. In the Fitzroy NRM region, it was assumed that only the breeders grazed P-deficient land types with three categories of P deficiency (marginal, deficient and acutely deficient), each with either (1) no P supplementation, or P supplementation during (2) the wet season, (3) the dry season, or (4) both the wet and dry seasons (i.e. 12 scenarios). Key results In the Katherine region, year-round P supplementation of the entire cattle herd (7400 adult equivalents) grazing acutely P-deficient pasture resulted in a large increase in annual business profit (+AU$500000). Supplementing with P (and N) only in the dry season increased annual business profit by +AU$200000. In the Fitzroy NRM region, P supplementation during any season of the breeder herd grazing deficient or acutely P-deficient pastures increased profit by +AU$2400–AU$45000/annum (total cattle herd 1500 adult equivalents). Importantly, P supplementation during the wet season-only resulted in the greatest increases in profit within each category of P deficiency, comprising +AU$5600, AU$6300 and AU$45000 additional profit per annum for marginal, deficient and acutely P-deficient herds respectively. Conclusions The large economic benefits of P supplementation for northern beef enterprises estimated in the present study substantiate the current industry recommendation that effective P supplementation is highly profitable when cattle are grazing P-deficient land types. Implications The contradiction of large economic benefits of P supplementation and the generally low adoption rates by the cattle industry in northern Australia suggests a need for targeted research and extension to identify the specific constraints to adoption, including potential high initial capital costs.

Nine insect species endemic to tropical America have been released into the Northern Territory as biological control agents for Mimosa pigra L. (Mimosaceae). To date, few studies have been published that quantify the abundance of these agents post‐release. Hence, populations of adult agents in three stands of M. pigra were monitored using Malaise traps. Trap catches frequently contained the lepidopteran agents Carmenta mimosa Eichlin and Passoa and Neurostrota gunniella (Busck). Neurostrota gunniella, which was first released in 1989, was the most abundant of all the agents caught, irrespective of the stand of M. pigra where trapping was conducted. The three coleopteran agents (namely Acanthoscelides Schönherr spp., Chlamisus mimosae Karren and Coelocephalapion Wagner spp.) were caught less frequently. Two recently released beetles, Chalcodermus serripes Fåhraeus and Sibinia fastigiata Clark, were not caught. Trap catches of Acanthoscelides spp. were greatest during the late dry season with smaller catches during the mid‐ to late wet season. Chlamisus mimosae was most abundant during the dry to dry–wet transition. Neurostrota gunniella was generally most abundant during the dry season. The abundances of Ca. mimosa and Coelocephalapion spp. exhibited little seasonality. Malaise trapping is recommended as part of a long‐term monitoring program to quantify agent populations and their impact upon M. pigra.

The performance of Brahman heifers at their first mating as yearlings (4-year groups of ~100 per year) grazing on improved pasture in the Douglas Daly region and as 2 year olds (3-year groups of ~100 per year) grazing on native pasture in the Victoria River District was studied. The relationships between pre-mating weight and pregnancy rate were established for Brahman heifers mated as yearlings on improved pasture, and as 2 year olds on native pasture with pre-mating weights recorded in late October/early November (before the wet season starts) and in late December (just before the start of mating). These relationships were found to be different indicating that there is an interaction between age and weight that modifies the effect of weight. The relationships were used to model pregnancy rates that are likely to result from different pre-mating weights for these three scenarios. These estimates can be used to identify target mating weights for different situations, and to predict the pregnancy rates for groups of heifers, which will be useful in budgeting and assessing the profitability of different management strategies.

The performance of heifers from a Brahman herd that was selected for fertility was compared with Brahman heifers sourced from commercial properties using three year-groups of heifers. Each year, conception rates from yearling mating were significantly higher in heifers from the selected herd (SEL) than in heifers sourced from commercial properties (COM), despite the average joining weight of the SEL heifers being lower each year. The pregnancy rate in SEL heifers was 24% higher (P = 0.008) in the first year, 36% higher (P = 0.005) in the second year and 45% higher (P < 0.001) in the third year. Over the 3 years of the study, the conception rate was 35% higher (P = 0.009) in SEL heifers than in COM heifers. Joining weight also affected pregnancy rates. When heifers were split into weight ranges according to their pre-joining weight, pregnancy rates were significantly higher (P = 0.018) in the heavier weight range than the lighter one. The results indicate that selection for fertility has been successful in improving conception rates from yearling mating in SEL heifers.