The study aimed at determining the farmers' knowledge of the pesticides and their use on the field in Cotton. Fifty items of knowledge on helath hazards of pesticides usage of cotton were selected from a large number of item pool collected based on standard criteria and in consultation with scientists of Regional Agricultural Research Station Tirupati, Subject Matter Specialists, Master Trainers of Acharya N.G. Ranga Agricultural University. Finally 26 items were selected after following the statistical procedures for the construction of standard knowledge test.

To establish the fingerprint profile of total extract of Caralluma fimbriata using high performance thin layer chromatography (HPTLC) technique. HPTLC studies were carried out. CAMAG HPTLC system equipped with Linomat V applicator, TLC scanner 3, Reprostar 3 and WIN CATS-4 software were used. HPTLC finger printing of Caralluma fimbriata leaf extract revealed that the chromatogram obtained with the reference solution shows in the middle part a Dark blue zone [Slimalumaside A] at RF 0.33 and in the upper part a Blue zone [Slimalumaside B] at RF 0.94 was observed. It can be concluded that HPTLC fingerprint analysis of total extract of Caralluma fimbriata can be used as a diagnostic tool for the appropriate identification various extracts.

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