The DNA templated self-assembly of gold nanoparticles clustered in different configurations (nn = 2–∞) was investigated in the colorimetric detection of ToLCNDV DNA using a gold nanoparticle conjugated bifunctional oligonucleotide probe.

Colletotrichum gloeosporioides is the most destructive endophytic plant-pathogenic fungi causing Anthracnose disease in a wide number of economically important plants throughout the world. By far there is no existing methods possessing an effective disease management over the outbreak of anthracnose disease and it's precede to huge economic losses for formers and plant tissue culture laboratories. In order to find a new and effective control measure over these endophytes, with aid of nanotechnology, we synthesized bioactive bile salt of sodium deoxycholate (NaDC) capped silver nanoparticles and satisfactorily characterized. Further it was employed to control the endophytic fungus through in vitro direct and indirect model system with time dependant/light mediated incubation manner. In our findings we achieved fivefold synergistic effect of NaDC-capped AgNPs with their bioactive capping agent against Colletotrichum gloeosporioides upon analyzing different parameters with typical instruments. Moreover, it was evident that NaDC-capped AgNPs did not cause any phytotoxicity to treated plants revealed by molecular markers. Figure: 6. Direct antifungal activity: FESEM images of Colletotrichum gloeosporioides incubated with NaDC capped AgNPs at various timings shows degrees of cell damage: (a) 0 min (b) 30 min (c) 60 min (d) 90 min (e) 120 min and (f) 150 min.

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