Bioactive bile salt-capped silver nanoparticles activity against destructive plant pathogenic fungi through in vitro system

Abstract

Colletotrichum gloeosporioides is the most destructive endophytic plant-pathogenic fungi causing Anthracnose disease in a wide number of economically important plants throughout the world. By far there is no existing methods possessing an effective disease management over the outbreak of anthracnose disease and it's precede to huge economic losses for formers and plant tissue culture laboratories. In order to find a new and effective control measure over these endophytes, with aid of nanotechnology, we synthesized bioactive bile salt of sodium deoxycholate (NaDC) capped silver nanoparticles and satisfactorily characterized. Further it was employed to control the endophytic fungus through in vitro direct and indirect model system with time dependant/light mediated incubation manner. In our findings we achieved fivefold synergistic effect of NaDC-capped AgNPs with their bioactive capping agent against Colletotrichum gloeosporioides upon analyzing different parameters with typical instruments. Moreover, it was evident that NaDC-capped AgNPs did not cause any phytotoxicity to treated plants revealed by molecular markers. Figure: 6. Direct antifungal activity: FESEM images of Colletotrichum gloeosporioides incubated with NaDC capped AgNPs at various timings shows degrees of cell damage: (a) 0 min (b) 30 min (c) 60 min (d) 90 min (e) 120 min and (f) 150 min.

In vitro and in vivo antifungal properties of silver nanoparticles against Rhizoctonia solani , a common agent of rice sheath blight disease

“…SNPs with chitin inhibit the spore germination of the examined pathogens [44, 45]. Moreover, bioactive capped SNPs were found to be able to control the endophytic fungus of C. gloeosporioides, in vitro [46]. The antifungal activity of SNPs is comparable with those of ionic SNPs; however, ionic silver remains cytotoxic at the concentrations that inhibit the growth of the examined yeasts [47].…”

Metal Nanoparticles as Novel Antifungal Agents for Sustainable Agriculture: Current Advances and Future Directions

“…In Table 1, the different extracts of plants and fungi that have been used to produce Ag nanoparticles are reported. In the case of the chemical route, several methods have been used to synthesize Ag nanoparticles, such as chemical reduction, sol-gel, and microemulsion [122][123][124][125][126][127][128][129][130]. To a lesser extent, physical methods have been used, such as high-voltage arc discharge and the irradiation method [131][132][133].…”

Multifunctional Properties of Commercial Bile Salts for Advanced Materials Engineering

“…[ 16 ] Biocompatible sodium deoxycholate‐modified Ag NPs were developed for antimicrobial applications. [ 17 ] Ag NPs modified with sodium deoxycholate were tested in agriculture for breaking seed dormancy. [ 18 ] Ag NPs synthesized and modified using sodium deoxycholate exhibited the plasmon‐induced chirality [ 19 ] due to the interaction of the adsorbed modifier molecules and plasmonic Ag NPs.…”