ObjectivesTo study relationship between serum levels of nicotinamide phosphoribosyltransferase and laboratory markers of inflammation in patients with rheumatoid arthritis (RA).MethodsWe determined nicotinamide phosphoribosyltransferase level in sera of 140 patients with RA (96 women and 44 men) by indirect enzyme-linked immunosorbent assay (RaiBiotech, cat No. EIA-VIS-1). The control group consisted of 20 women and 10 men aged 22 to 55 years without complaints of pain in the joints throughout life. The mean duration of disease was 5.94±0.37 years.ResultsWe divided all RA patients into 2 groups: one group (118 patients) with elevated levels of nicotinamide phosphoribosyltransferase serum (more than 3.9 ng/ml) and second group (22 patients) - with normal range.In each of the two groups, the levels of CRP and ESR were determined.Patients with elevated levels of Nampt had the following laboratory parameters (M±m): ESR – 37.83±1,57, CRP – 56.09±3,73 (rate - less than 5.0 mg/l). The second group had following data: ESR 22.46±0,56, CRP 21,65±1.38. Thus, patients with elevated levels of nicotinamide phosphoribosyltransferase had significantly higher concentrations of ESR and CRP (p<0,001).ConclusionsThere is the relationship between the level of nicotinamide phosphoribosyltransferase serum and laboratory markers of inflammation in RA (CRP and ESR). The data indirectly confirm the hypothesis that increased levels of nicotinamide phosphoribosyltransferase in RA patients is associated with disease activity.Disclosure of InterestNone declared

BackgroundFetuin-A is an acute-phase protein with contradictory effects. It is well known that fetuin-A low levels are associated with calcification and higher risk of cardiovascular diseases and its level is downregulated by pro-inflammatory cytokines.1 Nevertheles, it was shown, that fetuin-A induces synthesis of pro-inflammatory cytokines in adipocites and macrophages.2 ObjectivesTo investigate the level of fetuin-A in women with rheumatoid arthritis (RA).MethodsAt baseline we measured fetuin-A level, femoral neck, total hip and LI-LIV BMD by DXA in 110 women with RA (mean age 54,5±12,6; hereinafter M±Std.dev.) and 30 healthy controls. Serum CRP and ESR were measured to assess inflammation. DAS28 was calculated to determine RA activity. The diagnosis of osteoporosis was set according to the recommendations of world health organisation – T-score≤−2,5 for patients without glucocorticoid therapy in anamnesis, T-score≤−1,5 for patients treated with glucocorticoid for 3 months in anamnesis or with an osteoporotic fracture in anamnesis. Fetuin-A in serum was determined by enzyme-linked immunosorbent assay.ResultsMean concentration of fetuin-A in group with RA was 765,69±120,64 ug/ml, which was lower than of healthy controls – 812,95 ug/ml (p=0,0438). Secondary osteoporosis was revealed in 52 patients (47%) with RA with mean level of fetuin-A at 733,7±18,83 ug/ml vs. 794,36±12,83 ug/ml (p=0,0078) of 58 (53%) non-osteoporotic patients. Moderate negative correlations were observed between fetuin-A and DAS28 (r=-0,4334; p<0,001), fetuin-A and CRP (r=-0,3148; p<0,001), fetuin-A and ESR (p=-0,344; p<0,001). Mean concentrations of fetuin-A were significantly different between the subgroups with moderate (3,2≤DAS28<5,1) and high disease activity (5,1≤DAS28) of RA patients and healthy controls: 742,41±12,07 ug/ml vs. 812,95 ug/ml (p=0,0021) and 663,9±39,14 ug/ml vs. 812,95 ug/ml (p<0,001).ConclusionsOur study confirms that lower levels of fetuin-A are associated with higher activity of RA and with the loss of bone mineral density.References[1] Brylka L, et al. Calcif Tissue Int2012;93(4):355–364.[2] Stefan N, et al. Diabetes2008;57(10):2762–2767.Disclosure of InterestNone declared

Background:Attention is drawn to the frequent combination of osteoarthritis (OA) with cardiovascular disease. Non-specific inflammation plays a significant role in the pathogenesis of OA and atherosclerosis. Limiting the physical activity of patients with OA is an additional important factor aggravating the course of cardiovascular disease (CVD). Chronic pain syndrome, causing a neuroendocrine response, is often the cause of the development of complications of atherosclerotic disease. Dyslipidemia is the main cause of atherosclerosis and vascular thrombosis.Objectives:To study variants of lipid metabolism disorders in female and male patients of different age groups with osteoarthritis.Methods:Case histories of 90 patients with OA were analyzed. The average age of patients was 63.27 ± 11.31 years. The average body mass index (BMI) is 39.8 ± 3.2. All patients underwent questionnaires, general clinical and biochemical blood tests with lipid profile determination, anthropometry, bioimpedansometry, and the main metabolic rate assessment using indirect calorimetry in dynamics (at the beginning of the study and after 3 months).Results:Burdened heredity for obesity, arterial hypertension (AH), diabetes mellitus (DM) was revealed. AH was diagnosed in 76 patients (84.4%), type II diabetes in 17 (18.9%), dyslipidemia and hypercholesterolemia in 56 (62.2%). Statins were taken by 43 patients (47.8%) - group I patients, which is associated with low adherence to therapy, group II included patients who did not initially take statins or stopped taking them at least 6 months before inclusion in the study.Against the background of diet therapy and physiotherapy exercises, BMI (R0.99; p <0.05), fat mass (R0.95; p <0.05) significantly decreased, lipid profile normalization was noted: total cholesterol (R0.66; p <0 .05), LDL (R0.69; p <0.05), HDL (R0.95; p <0.05), TG (R0.57; p <0.05), AST decreased (R0.64; p <0.05) and ALT (R0.76; p <0.05) in both groups of patients, regardless of lipid-lowering therapy. A decrease in fat mass correlated with TG levels (R0.51; p <0.05), an increase in skeletal muscle mass (R0.60; p <0.05), lean mass (R0.72; p <0.05), and active cell mass (R0.59; p <0.05). The lipid profile in the I group of patients was significantly better before and at the end of the study. Long-term effects have not been investigated due to the short duration of the study.Conclusion:In patients with OA, a high frequency of concomitant diseases of the cardiovascular system, lipid metabolism disorders was found. Non-drug therapy has a positive effect on the lipid profile and the level of transaminases. The decrease in body weight due to loss of fat mass reliably correlates with the level of TG. Timely use of statins contributes to the normalization of the lipid profile, reduces the risk of cardiovascular disease in patients with OA. It is necessary to study lipid profile disorders in patients with OA with recommendations for lifestyle modification (diet, physical activity), and if necessary, prescribe lipid-correcting therapy.References:[1]E. Simakova, B. Zavodovsky, L. Sivordova [et al]. Prognostic significance of lipid disorders markers determination in pathogenesis of osteoarthritis. Vestnik Rossijskoj voenno-medicinskoj akademii. 2013. No. 2 (42). P.29-32.[2]Zavodovsky B.V., Sivordova L.E. Prognostic significance value of definition of leptin level determination in osteoarthritis. Siberian Medical Journal (Irkutsk). 2012; 115(8):069-072.Disclosure of Interests:None declared

There are significant emotional problems in patients with systemic sclerosis (SS) and rheumatoid arthritis (RA), including most expressed symptoms of anxiety and depression that complicate the process of social and psychological adaptation of patients, reduce the level of subjective control as an attribute of personal responsibility for controlling their disease. The effectiveness of the biofeedback (BFB) training inthe treatment of patients with rheumatoid arthritis and systemic sclerosis is analyzed. Analysis of the effectiveness of treatment was conducted by studying the dynamics of a number of psychological indices (the level of subjective control, reactive and personal anxiety, depression) and their comparison in patients of the main and control groups before and after the treatment. Correction of the psycho-emotional state of patients with SS and RA was carried out using biofeedback (BFB), based on the principle of self-regulation of body functions using external feedback systems. There was a significant reduction in anxiety and depressive reactions in patients, during the process of BFB therapy, accompanied by a significant decrease of scoresby Spielberger–Khanin and Beck psychological tests, noted a significant effect of increasing the level of subjective control that can improve the effectiveness of therapy and long-term disease prognosis. Thus, the additional application of BFB training in complex treatment of SS and RA patients promoted the improvement of the efficiency of the treatment and rehabilitation carried out and the improvement of the patients’ quality of life. Keywords: BFB training, systemic sclerosis, rheumatoid arthritis, rheumatic diseases, reactive anxiety, personality anxiety, depression, level of subjective control

Министерство науки и высшего образования Российской Федерации Федеральное государственное бюджетное научное учреждение «Научно-исследовательский институт клинической и экспериментальной ревматологии имени А.Б. Зборовского» Федеральное государственное бюджетное образовательное учреждение высшего образования «Волгоградский государственный медицинский университет» Министерства здравоохранения Российской Федерации