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Hybrid silica films containing cation‐exchange polyelectrolytes were designed and used to modify a glassy carbon electrode (GCE) for voltammetric determination of purine bases. Hybrid silica‐polyelectrolyte films synthesised in the presence of adenine as structure directed component have demonstrated enhanced sorption capacity to purine base. The anodic peaks of adenine and guanine at a hybrid film‐modified GCE were observed at +1.55 and +1.1 V, respectively, in phosphate buffer solution at pH 3.5. Oxidation currents of adenine and guanine were proportional to their concentration in the range of 0.02–0.50 mM, with a detection limit of 0.015 mM. The developed method was used to determine adenine in adenosine triphosphate and purine bases in hydrolyzed solutions of DNA and demonstrated good metrological characteristics.

Abstracts-The analytical prospects of a carbositall electrode modified with a film based on SiO 2 , hemoglo bin (Hb), and gold nanoparticles (CSE SiO 2 -Hb-Au), prepared by deposition with an electrically generated catalyst, are reported. Hemoglobin on the surface of CSE SiO 2 -Hb-Au is shown to possess electrocatalytic activity toward dissolved oxygen, giving a rationale for the development of a rapid voltammetric procedure of O 2 quantification with a detection limit of 0.1 mg/L. The inhibition activity of a number of nitrogen contain ing organic substances to the catalytic current CSE SiO 2 -Hb-Au is studied. A voltammetric procedure is proposed for determination of the anti flu drug rimantadine, 1 (1 adamantyl)ethylamine hydrochloride, with a detection limit of 0.4 mg/L. The procedure is applicable to the quantification of rimantadine in blood serum and saliva.