Salmonella typhi is gram-negative bacteria that caused typhoid fever in humans; prevention of the disease is currently through vaccination. The development of disease detection tools is also being carried out so that the detection process is faster and more accurate. In line with the development of typhoid detection devices, prior studies have managed to find factors that influence the production of Fim-C S. typhi protein on a small scale as raw material for typhoid detection kits. The purpose of this research is to apply the results of previous studies in the production of recombinant Fim-C-S. typhi proteins with volume variations of 50mL-300mL, as a foundation for large-scale production. The results of protein production were characterized by Sodium Dodecyl Sulfate Polyacrilamide Gel Electrophoresis (SDS-PAGE) and protein concentration measurements using the Bicinchoninic Acid (BCA) Assay at a wavelength of 562 nm. The results showed an amount of that protein increase along with gradually production volumes, mainly the protein in the form of inclusion bodies. According to the results obtained can be concluded production of the recombinant protein Fim-C-S. typhi at 50-300 mL volume variation on a laboratory scale has been successfully performed with consistent results, which is expected to be basic in production at pilot scale and large scale.

Typhoid fever caused by Salmonella typhi is an endemic disease in Asia, including Indonesia. Based on data from the World Health Organization (WHO), there are 81% per 100,000 people in Indonesia infected with typhoid. Based on this, development of typhoid detection kit that is simple, fast, specific and sensitive is still needed. One of the developments of detection kits currently available is the antigen and antibody interaction approach. This study aims to obtain comprehensive information about purification of recombinant Fim-C-S. typhi proteins as antigens for raw materials for manufacturing typhoid detection kits. This study uses the IMAC method with Ni2+ metal, which has advantages in high selectivity and purity. The results of this study report that purification of recombinant Fim-C-S. typhi protein inclusion bodies with variations in the binding and washing process at 3, 5, and 7 times gave percent yields correspondingly of 37.84, 39.44, and 38.21% pure protein. Previous studies have also reported variations in binding and washing at 2, 4, and 6 times and gave percent yields respectively were 35.37, 39.11, and 40.49%. Based on the data, we concluded that the variation of 6 times is the best repetition variation to get the largest percent yield so that it can be used as a reference in large-scale purification.