The medicinal plants have gained significant importance in traditional medicine and pharmacy due to wide variety of curative properties. The medicinal properties of the plants rely on the presence of various phytoconstituents. The present study focused on screening of phytochemicals from different parts (leaf, stem, petiole, fruit and root) of Solanum khasianum using solvents with varying polarity like methanol, butanol, chloroform, acetonitrile and water. The qualitative screening of phytoconstituents showed the existence of alkaloids, flavonoids, phenols, tannins, steroids, saponins and absence of quinones in all 25 extracts tested. The quantitative determination showed that root extract contains considerable amounts of total flavonoids (214.74±0.45), total phenols (183.47±0.38), total alkaloids (132.46±0.28), total antioxidants (95.66±0.27), total tannins (14.66±0.07) whereas total saponins were present in high content in fruit methanolic extract (44.99±0.33). The presence of these phytoconstituents indicates the therapeutic potential of S. khasianum and scientifically validates the traditional use of this plant.