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Abstract. In this paper, we adopt the QGWA-03 plant audio apparatus to investigate the sound effects on strawberry in the leaf area, the photosynthetic characteristics and other physiological indexes. It was found that when there were no significant differences between the circumstances of the two sunlight greenhouses, the strawberry after the sound wave stimulation grew stronger than in the control and its leaf were deeper green, and shifted to an earlier time about one week to blossom and bear fruit. It was also found that the resistance of strawberry against disease and insect pest were enhanced. The experiment results show that sound wave stimulation can certainly promote the growth of plants.

Agri-wave technology is a new agricultural technology based on the plant meridian system, that focuses on measurement of plant sound characteristics. The basic principle of agri-wave technology is to improve the yield and quality of plants such as vegetables, flowers, and fruit trees by broadcasting sound waves of certain frequencies and spraying a compound microelement fertilizer on the leaves. The application of agri-wave technology on tomatoes remarkably stimulates growth of their seedlings. Fresh weight of the branch, stems, and leaves of the treated tomatoes is significantly (59.53%, P < 0.0001) higher than that of the control group. Sampling survey results indicate that agri-wave technology accelerates the ripeness of tomatoes. The fresh weight of ripe tomatoes treated with this technique is 30.73% higher than that of the untreated (P = 0.0018), while the fresh weight of the treated unripe tomatoes is 27.29% lower than that of the untreated unripe group (P = 0.0020). Yield surveys show that the yield of treated plants is 13.89% (p < 0.0001) higher than that of the control group. Moreover, with agri-wave technology treatment the storage period of tomatoes is almost doubled. Analysis of tomato nutrition shows that agri-wave technology has increased their sugar content by 26.19%, vitamin A and niacin (an antifavours vitamin) by 55.39% and 92.31% respectively. There is no difference concerning vitamin B1, B2, and D content between the two groups, and vitamin C and E contents decreased by 2.10% and 12.69%, respectively. Among the analyzed 33 minerals of tomatoes, 26 increased in content, while 7 decreased. In conclusion, agri-wave technology has promoted the growth of the tomato, increased its yield, and improved its quality.

Studies on the sound characteristics of phylodendron performed by measuring the power of plant leaves with a laser beam found that the leaves of phylodendron could produce sound waves at relatively low frequencies (from 50 Hz to 120 Hz). Furthermore, it was found that those leaves could accept external sound wave stimulations, with frequencies lower than 150 Hz giving the strongest responses. When the plants were under stress, such as drought, the sound emissions from the plant's leaves increased approximately 20-30 dB, while the range of response to external sound wave stimulation decreased 10-20 dB. However, these increased emissions returned to normal six minutes after watering. When the stainless steel needles were inserted into the petiole of the plant, spontaneous sound production was increased about 40 dB for the main vein and 6 dB for the mesophyll. This is our third report on experimental evidence that plants might have a meridian system as in humans and other animals.

We have shown previously that plants exhibit functional characteristics similar to the meridian system in humans and animals, such as high potential and low electrical resistance, high temperature, and spontaneous sound production. Here we will show the effect of acupuncture on plants. When plants of Phaseolus vulgaris L. pole bean (cv. Kentucky wonder) and bush bean (cv. Slenderette) were subjected to acupuncture by inserting two needles into opposite sides of the stem of the unifoliolate buds, it was found that acupuncture strengthened the growth and development of the plants. Two repeated experiments showed that the mean net photosynthesis rate of plants subjected to acupuncture increased about 20.5%, the mean transpiration 27.2%, the growth and total length of internodes 22.5%, and the total dry weight of shoots from the cotyledon to the apex 22.9%, in comparisons with control plants under the same growing conditions. In addition, treated plants flowered three days earlier and had 14.4% more fruit than the untreated control plants. Thus, acupuncture may serve as a viable technique for increasing yield in agricultural plants.