Pineapple (Ananas comosus L. Merr) is one of Indonesia’s important fruit commodities. Indonesia became the world’s fourth-largest pineapple producer in 2020. The monoculture cultivation system can lead to the continuous presence of pests and diseases in plantation areas. Mealybug wilt disease is caused by Pineapple mealybug wilt-associated virus (PMWaV) and its thought to be associated with phytomatodes. This study aims to determine the type of phytomatodes in pineapple plants with three health degrees: healthy plants, moderate symptoms, and severe symptoms. The soil and roots were sampled at 3, 5, 13, and 14 MAP (Month after Planting). Nematodes were extracted from the soil using the flotation-centrifugation method, while the root samples were extracted using the mist chamber method. Identification of nematode morphology was based on anterior (mouth and stylet shape), posterior (tail shape and reproductive organs), and the type of annulation or nematode cuticle surface. Seven genera of phytomatodes were identified from soil and root samples: Pratylenchus spp., Rotylenchulus spp., Meloidogyne spp., Helicotylenchus spp., Crinonemoides spp., Tylenchus spp., and Rotylenchus spp.. The dominant phytonematodes in the soil samples were Rotylenchulus spp. (66.67/100 ml of soil), while Pratylenchus spp. predominated in the roots (58.33/10 g of roots). Based on the three degrees of plant health, the phytonematodes that dominated the soil samples were Rotylenchulus spp. on the health degree of healthy and moderate symptoms, and Meloidogyne spp. dominate the sample at severe symptoms. Pratylenchus spp. dominated the root samples at healthy and severe symptoms, while Rotylenchulus spp. predominate in moderate symptomatic samples.

Bean common mosaic virus (BCMV) and its aphid vector, Aphis craccivora, are essential limiting factors in yardlong bean production. The previous study showed that endophytic fungi (EF), Lecanicillium lecanii (LL), and H5 could suppress virus titer in BCMV-infected plants by its vector Aphis craccivora. Therefore, elucidation of its mechanisms is a necessary study. This research aimed to study the ability of aphids reared on EF-treated and untreated control plants to transmit BCMV. The adult aphids had been raised on EF-treated yard-long beans for three generations. The 3rd generation of adult aphids was used as vectors to transmit BCMV after acquisition feeding on infected plants and inoculating feeding on healthy plants. The disease assessment parameters were observed at four weeks post-transmission. Generally, the population number of aphids on EF-treated plants was significantly lower compared to the control. The transmission of BCMV using the 3rd generation of aphids reared on EF-treated plants affected by the prolonged incubation period, lower disease incidence and severity score, and significantly lower virus titer compared to control. Moreover, the detection of BCMV by ELISA in aphids after virus transmission showed an adverse reaction, indicating that the virus from acquisition feeding was transmitted to plants during inoculation feeding. These results revealed that rearing aphids on EF-treated plants interfere with the aphids’ ability to transmit BCMV by decreasing virus acquired during acquisition and inoculation feeding.