Hybrid surveys using hydroacoustic and fish sampling gears such as trammel net, trap were conducted to investigate total biomass, distribution and dominant fish species of the demersal fishes in the marine ranching area (MRA) of Jeju. Four surveys were carried out in June, August, October and November using 38kHz quantitative echo sounder. Catch data using trammel net and trap were used to calculate biomass and to examine dominant fish species. Fish schools were mainly detected in the waters of 20 meters below and around Chagwido waters. By the result of fishing experiments, fish species living in MRA of Jeju were about 40 species, dominant fish species of a detectable fish such as Family Sparidae and Family Monacanthidae etc. were identified 59.4~68.8% of total biomass. Based on the hydroacoustic data, fish length-weight function and target strength information, mean density of the demersal fish estimated were as

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This study monitored a variety of marine fish communities in artificial reefs unit of the total 5 types (Dice type, Octagonal turtle type, Two-stage tube type, Gazebo type, Tetrapod type) which are located in the marine ranching at Jeju island by scuba diving in May, July, October and December 2009. Underwater photographing was accomplished at total 3 phases (condition of artificial reefs photographing, concentric circle movement photographing and line transect photographing). The preservation condition of artificial reefs facility was very good, and the dominant species were Chromis notatus, Sebastes thompsoni, Oplegnathus fasciatus and Halichoeres poecilopterus. Fish abundance was high in May and June, and low in October and December, 2009. Chromis notatus was dominant at the all types of artificial reefs, Halichoeres poecilopterus for Gazebo type and Tetrapod type of artificial reefs, and Sebastes thompsoni for Dice type, Octagonal turtle type and Two-stage tube type of artificial reefs.

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Background: Marine invertebrates are well known as pivotal bioresources with bioactive substances such as antiinflammatory sterols, antitumor terpenes, and antimicrobial peptides. However, there are few scientific reports on chemical compositions and bioactivities of marine invertebrates from the East Sea of South Korea. Methods: In this study, chemical compositions and biological activities were evaluated on both 70% EtOH and hot water extracts of 5 species of marine invertebrates (Crossaster papposus japonicus, Actinostola carlgreni, Stomphia coccinea, Actinostola sp., and Heliometra glacialis) collected from the East Sea of South Korea. The antioxidant activities were measured by ABTS radical scavenging assay. The cytotoxicity and anti-inflammatory activity were evaluated using MTT and Griess reagents. Moreover, the antibacterial effect was evaluated using paper disc assay and minimum inhibitory concentration (MIC) assay. Results: In the results of antioxidant activities, 70% EtOH extract of A. carlgreni showed the highest activity (IC 50 0.19 ± 0.03 mg/ml) compared to other extracts. Moreover, 70% EtOH extract of A. carlgreni could significantly suppress the nitric oxide (NO) production in lipopolysaccharide-induced RAW 264.7. All extracts treated under 400 μg/ml have no cytotoxic effects on RAW 264.7 macrophages. In the antibacterial test, both 70% EtOH extracts of C. papposus japonicus and H. glacialis showed a significant antibacterial effect on Staphylococcus aureus. The MIC values were evaluated at 256 and 512 μg/ml, respectively. Conclusions: These results suggested the bioactive potentials of marine invertebrates from the East Sea of South Korea in pharmaceutical and nutraceutical applications.