There is a large amount of sulfur compound in crude oil, sulfur gases such us H2S for example are emitted and can affect the environment and finally can cause problems during the processing operation of crude oil. Therefore, in this study, a method for the removal of sulfur from crude oil using an ultrasound device has been proposed. This method is a combination of oxidative method and applying ultrasonic waves to achieve the allowable sulfur content in crude oil. Samples of crude oil have been taken that we got from an al-Najaf refinery to study the effect of three parameters (activated carbon weight, hydrogen peroxide volume, and acetic acid volume) at 50oC on the desulfurization process. the optimum result of desulfurization has been found to be (81.325%) at activated carbon amount of (9gm) with (H2O2/acetic acid)(40/30)ml.