Highlights d The SWR chromatin remodeler engages stochastically on either side of a +1 nucleosome d Temperature determines whether SWR inserts H2A.Z preferentially on one side of the nucleosome d The sequence of a 16-bp region affects temperaturedependent H2A.Z insertion d Nucleosomes with consecutive G/C bases are more H2A.Z enriched at yeast promoters

VivosX is an in vivo disulfide crosslinking approach that utilizes a pair of strategically positioned cysteines on two proteins to probe physical interactions within cells. Histone H2A.Z, which often replaces one or both copies of H2A in nucleosomes downstream of promoters, was used to validate VivosX. Disulfide crosslinks between cysteine-modified H2A.Z and/or H2A histones within nucleosomes were induced using a membrane-permeable oxidant. VivosX detected different combinations of H2A.Z and H2A within nucleosomes in yeast cells. This assay correctly reported the change in global H2A.Z occupancy previously observed when the deposition and eviction pathways of H2A.Z were perturbed. Homotypic H2A.Z/H2A.Z (ZZ) nucleosomes accumulated when assembly of the transcription preinitiation complex was blocked, revealing that the transcription machinery preferentially disassembles ZZ nucleosomes. VivosX works in human cells and distinguishes ZZ nucleosomes with one or two ubiquitin moieties, demonstrating that it can be used to detect protein-protein interactions inside cells from different species.

This paper mainly studies the apparent effects of the music played by Chinese traditional instrument Guzheng on biological functions, especially on heart rate and mood. Today, many scholars are seeking other active interventions, such as psychology, to reduce the psychological factors caused by the disease. Among them, more and more use music therapy. The author preliminarily evaluates the effect of the music played by Guzheng on people's physical and mental improvement. The evidence mainly comes from heart-rate measurement and questionnaires. The study finds that for Chinese people aged 15-17, Chinese Guzheng music has a postive effect on heart rate and mood.

No abstract

No abstract