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A Gram-positive bacterium, designated strain Kis4-19 T , was isolated from the intestinal tract of a fish, and its taxonomic position was investigated by a polyphasic approach. The cells of strain Kis4-19 T were coccus-shaped, non-motile and non-sporulating. The peptidoglycan type of this organism was A3; LL-diaminopimeric acid (LL-A 2 pm) was the diagnostic diamino acid of the peptidoglycan. The predominant menaquinone was MK-8(H 4 ), and the major fatty acids were iso-C 15:0 , iso-C 14:0 and iso-C 16:0 . Galactose was detected as a cell-wall sugar. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and lyso-phosphatidylethanolamine. The DNA G+C content was 71.1 mol%. Sequencing analysis of the 16S rRNA gene indicated that strain

Serinibacter salmoneus gen. nov., sp. nov., an actinobacterium isolated from the intestinal tract of a fish, and emended descriptions of the families Beutenbergiaceae and Bogoriellaceae ). In addition, on the basis of 16S rRNA gene sequence analysis of the genus Serinibacter and related genera, emended descriptions of the families Beutenbergiaceae and Bogoriellaceae are proposed to accommodate the genera Beutenbergia, Salana and Serinibacter, and the genera Bogoriella and Georgenia, respectively. 2009). It has been reported that the genus Ruania is phylogenetically related to the genera Georgenia, Bogoriella (Groth et al., 1997), Beutenbergia and Salana, but the family to which the genus Ruania belongs has not been specified (Gu et al., 2007).Members of the suborder Micrococcineae are widespread and occur in both natural and artificial habitats, e.g. in soil, plants, sediments, food and dairy products. While isolating bacteria from marine samples, we isolated a novel actinobacterium (strain Kis4-28 T ) from the intestinal tract of a fish species collected from Tokyo Bay, Japan. Comparative 16S rRNA gene sequence analysis revealed that strain T is a member of the suborder Micrococcineae. The objective of this study was to determine the taxonomic position of this strain by using a polyphasic approach.Japanese sillago (Sillago japonica), a type of fish, was collected from Kyonan beach on the coast of Tokyo Bay, Japan. The sillago was dissected, and an intestinal tract sample of approximately 1 g was used for the isolation of bacteria. The procedure employed for bacterial isolation was as described by Iino et al. (2007). NBRC medium 802 containing 1.0 % Polypepton (Wako), 0.2 % yeast extract (Difco), 0.1 % MgSO 4 . 7H 2 O and 1.5 % agar (if required) was used for general laboratory cultivation, morphological studies and for determining the optimal growth para-The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain T is AB455532.A phylogenetic tree derived from 16S rRNA gene sequences of strain Kis4-28 T and its taxonomic neighbours, including many strains in the suborder Micrococcinae, is available as a supplementary figure with the online version of this paper.