The thermoelastic effect was used to produce high-frequency, broadband ultrasound in water. A pulsed diode laser, followed by an erbium-doped fiber amplifier, was focused onto a light-absorbing film deposited on a glass substrate. Conversion efficiency was improved by over 20 dB using an elastomeric film instead of a more commonly used metallic one. Radiation pattern measurements show that considerable energy is radiated at +/−45° for frequencies beyond 50 MHz. These results show that the thermoelastic effect can be used to produce phased arrays for high-frequency ultrasound imaging.

Optical detection of ultrasound is a promising technique for high frequency imaging arrays. Detection resolution approaches the optical resolution, which can be on the order of the optical wavelength. We describe here an optical technique for ultrasound detection based on a thin ͑10 m͒ Fabry-Perot étalon optimized for high resolution imaging. The signal to noise ratio ͑SNR͒ approaches that of an ideal piezoelectric transducer over a 100 MHz bandwidth. Array functionality is demonstrated by scanning a probe beam along a line. Thermoelastic excitation was applied to generate acoustic waves in a test phantom containing a single "pointlike" source. An image of the source was reconstructed using signals acquired from the étalon detector array.

Photoacoustic microscopy (PAM) provides high resolution images with excellent image contrast based on optical absorption. The compact size and high repetition rate of pulsed microchip lasers make them attractive sources for PAM. However, their fixed wavelength output precludes their use in spectroscopic PAM. We are developing a tunable optical source based on a microchip laser that is suitable for spectroscopic PAM. Pulses from a 6.6 kHz repetition rate Q-switched Nd:YAG microchip laser are sent through a photonic crystal fiber with a zero dispersion wavelength at 1040 nm. The highly nonlinear optical propagation produces a supercontinuum spectrum spanning 500-1300 nm. A tunable band pass filter selects the desired wavelength band from the supercontinuum. Our PAM system employs optical focusing and a 25 MHz spherically focused detection transducer. En-face imaging experiments were performed at seven different wavelengths from 575 to 875 nm. A simple discriminant analysis of the multiwavelength photoacoustic data produces images that clearly distinguish the different absorbing regions of ink phantoms. These results suggest the potential of this compact tunable source for spectroscopic photoacoustic microscopy.

Two-dimensional phased arrays for high frequency (>30 MHz) ultrasonic imaging are difficult to construct using conventional piezoelectric technology. A promising alternative involves optical detection of ultrasound, where the array element size is defined by the focal spot of a laser beam. Element size and spacing on the order of a few microns are easily achieved, suitable for imaging at frequencies exceeding 100 MHz. We have previously shown images made from a receive-only, two-dimensional optoacoustic array operating at 10 to 50 MHz. The main drawback of optical detection has been poor sensitivity when compared with piezoelectric detection. In this paper, we explore a different form of optical detection demonstrating improved sensitivity and offering a potentially simple method for constructing two-dimensional arrays. Results from a simple experiment using an etalon sensor confirm that the sensitivity of etalon detection is comparable with piezoelectric detection. This paper concludes with a proposal for a high frequency optoacoustic array system using an etalon.

Background Looping is a crucial phase during heart development when the initially straight heart tube is transformed into a shape that more closely resembles the mature heart. Although the genetic and biochemical pathways of cardiac looping are well-studied, the biophysical mechanisms that actually effect the looping process remain poorly understood. Using a combined experimental (chick embryo) and computational (finite element modeling) approach, we study the forces driving early s-looping when the primitive ventricle moves to its definitive position inferior to the common atrium. Results New results from our study indicate that the primitive heart has no intrinsic ability to form an s-loop and that extrinsic forces are necessary to effect early s-looping. They support previous studies that established an important role for cervical flexure in causing early cardiac s-looping. Our results also show that forces applied by the splanchnopleure cannot be ignored during early s-looping and shed light on the role of cardiac jelly. Using available experimental data and computer modeling, we successfully developed and tested a hypothesis for the force mechanisms driving s-loop formation. Conclusions Forces external to the primitive heart tube are necessary in the later stages of cardiac looping. Experimental and model results support our proposed hypothesis for forces driving early s-looping.