Mutations occur as a result of alterations in DNA or during the replication/cell division process. For agricultural development, plant breeding necessitates genetic variety of valuable features. Multiple mutant alleles, on the other hand, constitute a source of genetic variety for crop breeding and, in many cases, functional investigation of the targeted gene. Plant breeding can only improve when the breeder has access to enough variation for a particular trait. Any change in an organism's DNA that is not caused by normal recombination and segregation is referred to as a mutation. Exposure to mutagenic agents such as radiation or certain chemicals, as well as faults made during normal cell division and replication, are all possible causes. The first breeding successes were achieved by utilizing spontaneous (naturally occurring) mutations. The most well-known example is the use of semi-dwarf wheat and rice mutants during the 'Green Revolution.' Induced mutagenesis is becoming increasingly popular in plant molecular biology as a method for identifying and isolating genes, as well as studying their structure and function. Molecular mutation breeding is ushering in a new era of crop enhancement mutation breeding. In the coming years and decades, mutation breeding will play a vital role in crop improvement and resolving concerns related to global food security. As a result, the goal of this review paper is to evaluate the function of mutant breeding in crop development and how it might be used.