Lathyrus sativus L. is considered one of the most promising calorie and protein sources for the vast, expanding populations living in drought-prone and marginalized areas of the world. This study was conducted to determine the β-N-oxalyl-L-2,3-diaminopropionic acid (β-ODAP), L-homoarginine, and asparagine content of a total of 173 Lathyrus sativus L. genotypes, of which 93 were collected under natural conditions in Antalya. The β-ODAP, L-homoarginine, and asparagine content of Lathyrus sativus L. genotypes ranged from 1.55 to 20.8 mg/g seeds, 1.35 to 11.64 mg/g seeds, and 0.59 to 5.22 mg/g seeds, respectively. ARTICLE HISTORY

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Residues in animal feeds and foods of animal origin have been important safety issue concerning both human and animal health. A multiresidue method for determination of eight mycotoxins and ten antibiotics was developed and validated in animal feeds by using QuEChERS (quick, easy, cheap, effective, rugged, and safe) extraction followed by UHPLC-MS/MS. Optimisation of UHPLC-MS/MS parameters was performed to achieve good separation and resolution. The method was validated according to the European Commission Decision 2002/657/EC. Matrix matched calibration curves showed good r2 (≥0.995) values, and limit of quantification (LOQ) values varied between 1.2 and 5.2 μg kg−1. Average recoveries ranged from 60 to 102% with relative standard deviations of 2.2 and 15.6% for all type of feed samples except for tetracyclines, lincomycin, tylosin, ochratoxin A, and fumonisin (B1 and B2).

Background: Considering global warming and climate changes, grass pea (Lathyrus sativus L.) is becoming advantageous and popular with its characteristics such as resistance to biotic stresses. The objective of the present study was to determine the variations in fatty acid and organic acid content in induced grass pea populations. Methods: Forty-one grass pea genotypes that include forty mutant genotypes and one released variety (Gürbüz 2001) were investigated in term of fatty acids using the GC-MS device and organic acids using UPLC-MS/MS device. Result: Heptadecanoic acid, stearic acid, oleic acid, linoleic acid and α-linolenic acid were determined as major fatty acid constituent in forty mutant genotypes. In saturated fatty acids, the highest values were determined as 403.24, 401.39 and 398.87 mg 100 g-1 (in GPM5, GPM20, GPM34), respectively. Compared the control cultivar, the maximum values of GPM21 (18.77%), GPM5 (18.68%) and GPM33 (18.12%) were higher in PUFA. And in the similar, the values of GPM19 (11.52%), GPM28 (10.54%) and GPM4 (5.62%) were lower obtained in PUFA. As the maximum values of succinic, lactic, oxalic, citric and tartaric acid were obtained in GPM9, GPM37 and GPM11, the lowest values were found in GPM4, GPM13 and GPM18, respectively. The values of fatty acids and organic acids, important criteria for animal feeding and human nutrition, will guide further breeding studies for the development of these 40 mutant genotypes.