Small particle reagent technique is a method which is used to develop fingermarks on moist and smooth surfaces. It consist the theory of adherence of fine particles which is suspended in a treating solution to the oily or fatty components (water insoluble) present in in latent fingermark residue and gives a grey colored deposit. It is also considered as belonging to the family of powder dusting. The effectiveness of this technique can be increased by using fluorescent dyes in it's treating solution which can also detect faint fingermarks on multi-colored surfaces. Small particle reagent involves the use of synthetic detergent which was here replaced by saponin extracted from the fruit of Sapindus mukorossi as natural detergent. The other ingredient used in the formulation is black charcoal powder. This composition develops sharp and clear fingermarks on non-adsorbent surfaces like glass, metal sheet, plastic, laminated wood, ceramic tiles and compact discs after these were immersed in water for variable periods of time. The ability of the present formulation to detect weak chance prints not only enhances its utility, but also its potentiality in forensic investigations. The raw materials used to prepare the SPR are easily available and cost-effective.
Ninety per cent of the immune system resides in the gut and improvement in the gut health may lead to reduced incidence of infection. Hence the present study was undertaken to evaluate Fructooligosaccharide (FOS) supplementation on gut health impacting diarrhoeal morbidities, common cold and nutritional status of school going children (SGC). A randomized clinical trial was used to study 60 undernourished SGC who were randomly stratified into placebo (PG) and experimental group (EG). Incidence of diarrhoea and common cold were recorded for one month and during intervention. PG and EG were supplemented with plain sugar ice cream and FOS incorporated ice cream (100 ml) for 30 days respectively. Lactic acid bacteria; <em>Bifidobacteria</em> and <em>E.coli</em> in stool samples were analyzed using standard methods. Children who consumed ice cream with FOS showed significant improvement in the mean log counts of beneficial gut microbiota i.e. <em>Bifidobacteria</em> and Lactic acid bacteria by 29% (p=0.000) and 2.56% (p<0.04) respectively, along with 2.38% (p=0.000) reduction in <em>E.coli</em>. Diarrhoeal episodes were significantly lowered by 79.6% in EG. Common cold was reduced by 82.38% (p=0.000) in EG and 57.86% (p=0.000) in PG. FOS intervention however did not show any improvement in the anthropometric measurements. Daily consumption of 10 g of FOS for 30 days effectively enhanced the counts of <em>Bifidobacteria</em> and Lactic acid bacteria in the gut and may have impacted significant reduction in diarrhoeal episodes and counts of <em>E.coli</em> in SGC of low income families.
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