In this article, a novel linear mmWave antenna array with series‐feed network is proposed to enhance the bandwidth and reduce sidelobe level without increasing the patch size. The proposed linear array is consisted of four identical wideband array elements, which are all under operation TM10 and TM02 modes by loading shorting pin and rectangular slots. Additionally, through loading symmetry circle‐shaped slots for the four elements, impedance matching of linear array is achieved. Furthermore, multi‐parameters unified‐optimization (MPUO) based on imperial competition algorithm (ICA) is proposed to uniformly optimize all linear array parameters. To verify this design, the proposed linear array is fabricated with a small patch area of 7.5 × 3.914 × 0.254 mm3. The measured results show that the bandwidth is enhanced to 2.05GHz, which is 0.57GHz wider than that of simulation. The simulated peak gain reaches 13dBi while the sidelobe level is reduced to about −19 dB at 28.6GHz. Moreover, the computation cost using MPUO is reduced by 98.12% compared with that of independent parameters optimization.
Background
Dye-decolorizing peroxidases (DyPs) represent a novel family of heme peroxidases that use H2O2 as the final electron acceptor to catalyze the oxidation of various organic compounds. A DyP from Irpex lacteus F17 (Il-DyP4, corresponding to GenBank MG209114), obtained by heterologous expression, exhibits a high catalytic efficiency for phenolic compounds and a strong decolorizing ability toward various synthetic dyes. However, the enzyme structure and the catalytic residues involved in substrate oxidation remain poorly understood.
Results
Here, we obtained a high-resolution structure (2.0 Å, PDB: 7D8M) of Il‑DyP4 with α-helices, anti-parallel β-sheets and one ferric heme cofactor sandwiched between two domains. The crystal structure of Il‑DyP4 revealed two heme access channels leading from the enzyme molecular surface to its heme region, and also showed four conserved amino acid residues forming the pocket for the conversion of hydrogen peroxide into the water molecule. In addition, we found that Trp264 and Trp380, were two important residues with different roles in Il‑DyP4, by using site-directed mutagenesis and an electron paramagnetic resonance (EPR) study. Trp264 is a noncatalytic residue that mainly is used for maintaining the normal spatial conformation of the heme region and the high-spin state of heme Fe3+ of Il‑DyP4, while Trp380 serves as the surface-exposed radical-forming residue that is closely related to the oxidation of substrates including not only bulky dyes, but also simple phenols.
Conclusions
This study is important for better understanding the catalytic properties of fungal DyPs and their structure–function relationships.
In this paper, a novel wideband circularly polarized (CP) millimeter wave (mmWave) microstrip antenna is presented. The proposed antenna consists of a central patch and a microstrip line radiator. The CP radiation is achieved by loading a rectangular slot on the ground plane. To improve the 3-dB axial ratio bandwidth (ARBW), two symmetric parasitic rectangular patches paralleled to a central patch and a slit positioned to the right of the central patch are loaded. To verify this design, the proposed antenna is fabricated with a small antenna of 2.88 × 3.32 × 0.508 mm3. The measured impedance bandwidth (IMBW) for S11<−10 dB of the proposed antenna is 35.97% (22.8 to 33.8 GHz). Meanwhile, the simulation result shows that the 3-dB ARBW is 15.19% (28.77 to 33.5 GHz) within impedance bandwidth, and the peak gain is from 5.08 to 5.22 dBic within 3-dB ARBW. The proposed antenna is suitable for CP applications in the Ka-band.
A wideband differential-fed microstrip patch antenna based on radiation of three resonant modes of TM12, TM30, and slot is proposed in this paper. Firstly, two symmetrical rectangular slots are cut on the radiating patch where the zero-current position of the TM30 mode excites another resonant slot mode. In addition, the slot’s length is enlarged to decrease the frequency of the slot mode with little effect on that of the TM30 mode. To further expand the impedance bandwidth, the width of patch is reduced to increase the frequency of the TM12 mode, while having little influence on that of the TM30 and slot modes. Moreover, a pair of small rectangular strips is adopted on the top of the feeding probes to achieve a good impedance matching. Finally, based on the arrangements above, a broadband microstrip patch antenna with three in-band minima is realized. The results show that the impedance bandwidth (Sdd11<−10 dB) of the proposed antenna is extended to 35.8% at the profile of 0.067 free-space wavelength. Meanwhile, the proposed antenna maintains a stable radiation pattern in the operating band.
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