The research characterization of cytotoxic fraction against P-388 leukemia murine cells from the extract honje (Etlingera elatior) seed have been reported. This research lead to isolated and characterization of cytotoxic compounds against P-388 leukemia murine cells from the extract E. elantior seed. The extract of E. elantior seed was maserated by methanol, n-hexane, and ethyl acetate, respectively and estimated their cytotoxic activity against P-388 leukemia murine cell with 3- (4, 5-dimetiltiazol-2-yl) -2,5-difeniltetrazolium bromide (MTT) assay guided toxicity test against of shrimp Artemia salina Leach. Brine shirmp Lethality Test (BSLT) method. The active extracts will be separated by fractionation using column chromatography, radial chromatography, and for analyzing the purity of isolate will estimate by HPLC. The chemical structure of pure isolate will be identified by spectroscopies data UV Vis, FTIR, NMR and MS. The ethyl acetate extract from honje seed have cytotoxic activity by leukemia P-388 cell  with IC50 19.21 µg/mL. The compound toxic as cytotoxicagainst P-388 leukemia murine cells is flavonoid compouds their is resveratrol, lapachol, apigenin, methylated chrysin, 6,2’-dihydroxyflavanone, 3-hydroxy-3,4’-dymethoxyflavone and 4’-hydroxy-5,7-dimethoxyflavanone.DOI: http://dx.doi.org/10.15408/jkv.v0i0.3640

Active compounds with antioxidant activity were isolated from ethyl acetate extract of namnam stem (C. cauliflora L.) that had undergone maceration and fractionation by gravity column chromatography. The compounds were later identified by by using UV-Vis Spectrophotometry, FTIR, LCMS and 1H-NMR. Ethyl acetate extract of namnam stem showed considerably high antioxidant activity (IC50 value 4.68 ± 0.035 ppm). The results of analysis by UV-Vis and FTIR showed carbonyl group conjugated with an aromatic ring at band I (λmax 330.22 nm), chromophore group of alkene (C=C) at band II (λmax 268.67 nm) and functional groups such as O−H (3343.91 cm-1), C=O (1729.23 cm-1), C=C (1652.64 and 1611.99 cm-1), C−O (1269.89) and C−H ortho (738.23 cm-1). LCMS (m/z 270.9246) and 1H-NMR data showed seven proton signals on the aromatic ring at carbon position C-3 at δH 6.86 ppm (1H, s), C-6 at δH 5.95 ppm (1H, d, J=1.95 Hz), C-8 at δH 6.25 ppm (1H, d, J= 1.95 Hz), C-2’ and C-6’ at δH 7.03 ppm (2H, d, J=7.87 Hz), C-3’ and C-5’ at δH 6.87 ppm (2H, d, J= 7.87 Hz) so that the structure was identified as a flavonoid which was 4 ', 5,7-trihydroxy-flavones or known as apigenin. The isolated apigenin had very strong antioxidant activity, as shown by IC50 value of 5.18 ± 0.014 ppm.

Plants have compounds class of secondary metabolites that can be utilized as an antioxidant. Gandaria (Bouea macrophylla Griff) is one of the native plants of Indonesia that can be used as a source of antioxidant compounds. This study aims to test the antioxidant activity of B. macrophylla plant stem through DPPH method. B. macrophylla stem samples were cleaned, dried, and mashed. The fine sample of stem B. macrophylla was gradually extracted with n-hexane, ethyl acetate, and methanol. Each extract tested total phenolic and total flavonoids and tested antioxidant activity with 2,2-diphenyl-1 picrylhydrazyl (DPPH) Free Radical Scavenger method. The results showed that ethyl acetate extract had the best antioxidant activity of IC 50 4.89 µg/mL, with the total value of phenolic and flavonoid of 22.62 mg GAE/g and 32.28 mg quercetin/g.

Ethyl acetate extract of gandaria (Bouea macrophylla Griff) has very strong antioxidant activity. The aims of this study are to separate and characterize the active fraction of antioxidants and determine the antioxidant activity quantitatively. Ethyl acetate extract of B. macrophylla was separated by gravity column chromatography. Fraction separating was guided with qualitative testing of antioxidant activity. Active fraction result of separation was characterized by liquid chromatography-mass spectroscopy and quantitatively analyzed antioxidant activity using the 2.2-diphenyl-1-picrylhydrazyl method. Naringenin and luteolin were identified in the D-2 fraction which had very strong antioxidant activity with an IC50 value of 2.13 ppm. The hydroxyl group OH group of naringenin and luteolin compounds was thought to play a role in the reduction of free radicals.

Yellow frangipani flowers (Plumeria alba) are plants that commonly found in the tropics, including Indonesia. Frangipani utilization has not optimum and research about it has not many conducted. This study aims to characterize and test the antioxidant activity of frangipani flower extract. The ex traction conducted by maceration method using n-hexane, ethyl acetate, and methanol as solvents. Characterization includes phytochemicals assay (alkaloids, flavonoids, terpenoids, phenolic hydroquinone, tannin-polyphenols, and saponins), total phenolic assay, and total flavonoid assay. Antioxidant test using DPPH radical scavenging assay. The results of the characterization showed that frangipani flower extract positively contained alkaloid, flavonoids, tannins compounds in all solvent phases. Terpenoids are positive in n-hexane and ethyl acetate extract, while saponins are positive in methanol extract. The total phenolic content of n-hexane, ethyl acetate, and methanol extract were 3.6 mgGAE/g; 27.74 mgGAE/g; and 35.23 mgGAE/g. The total flavonoid content of n-hexane, ethyl acetate, and methanol extract were respectively 0.8 mgQE/g; 12.18 mgQE/g; 19.9 mgQE/g. The strongest antioxidant activity is possessed by ethyl extract, followed by methanol extract, and the last are n-hexane extract.