RESUMOO projeto de extensão BioquímicAtiva surgiu com o intuito de utilizar a internet como um meio de ensino da Bioquímica, dada a dificuldade que os alunos comumente relatam sobre a compreensão dessa disciplina e sua associação com a prática profissional. Por essa razão, o presente trabalho avaliou a eficiência de um blog educativo sobre Bioquímica, elaborado a partir de um post de fácil linguagem, didático e interativo, utilizando-se da interdisciplinaridade com a Fisiopatologia. Toda a criação do blog e de seu conteúdo foi executada pelos integrantes do projeto, os quais se basearam na literatura científica para a criação do post "Você Sabe o Que É Esteatose Hepática Alcoólica?". Para a avaliação do blog consideraram-se parâmetros de visão geral e informações demográficas, gerados pelo Google Analytics. O conteúdo postado foi avaliado pela enquete "O Que Você Achou Deste Conteúdo?" -disponível ao final do post para votação. O blog foi avaliado ao longo de quinze dias, contados a partir da data de sua divulgação; com registro total de 1236 visualizações nesse período, incluindo usuários de diversas cidades do Brasil e de outros países. Foram notificados 26 votos, sendo 25 para a opção "Excelente" (96,15%), um para "Bom" (3,84%) e nenhum voto para "Indiferente". Avalia-se que o projeto elaborado foi positivo, o que demonstra a importância do uso de novos métodos de ensino e tecnologias que possam contribuir para a formação e o interesse pela Bioquímica, bem como estimular seu uso para outros cursos de graduação, a fim de melhorar o processo de ensino e aprendizagem dos acadêmicos.Palavras-chave: Blog Educacional; Disciplina de Bioquímica; Interdisciplinaridade; Nutrição. ABSTRACTThe project of extension BiochemicalActive came with the intention of using the internet as a means of teaching Biochemistry, given the difficulty that students commonly report about the comprehension of this discipline and its association with practice. For this reason, the present study evaluated the effectiveness of an educational blog about Biochemistry, elaborated from a post of easy language, didactic and interactive using the interdisciplinarity with the Pathophysiology. All the creation of the blog and its content was executed by the members of the project, who based on the scientific literature to create the post "Do You Know What Is Alcoholic Liver Steatosis?". For the evaluation of the blog it was considered parameters of overview and demographic information, generated by Google Analytics. The content posted was evaluated by the poll "What Did You Think Of This Content?" available at the end of the post for voting. The blog was evaluated for 15 days, counted from the date of its disclosure, with a total of 1236 views in this period, including users from several Brazilian cities and some countries. "Good" (3.84%) and no votes to be indifferent. Our evaluation is that the project was positive, which demonstrates the importance of using new teaching methods and technologies that can contribute to the formation and interest in B...

Objetivo: Esse estudo objetivou caracterizar a disciplina de Nutrição Esportiva nos cursos de Graduação em Nutrição e Educação Física das Instituições de Ensino Superior do Centro-Oeste do Brasil e Distrito Federal. Material e Métodos: Adotou-se como critério de inclusão os cursos presenciais ou de educação à distância de Graduação em Nutrição (Bacharelado) e Educação Física (Bacharelado e Licenciatura) das Instituições de Ensino Superior reconhecidas pelo Ministério da Educação. Foram analisados os três estados que compõem a Região Centro-Oeste do Brasil e o Distrito Federal. As Instituições foram selecionadas através da Base de Dados do Sistema de Regulação do Ensino Superior – Sistema e-MEC e para a avaliação considerou-se a presença da disciplina Nutrição Esportiva e sua caracterização de oferta como sua denominação; natureza (obrigatória ou optativa); carga horária e período ministrado. Resultados: Observou-se que para 100% dos cursos de Nutrição a disciplina é de natureza obrigatória, ao passo para que para a Educação Física esse percentual foi de 95,23%. Ambos os cursos apresentaram ampla variabilidade da carga horária total e maior carga horária teórica, quando comparada à carga horária prática. Também, alguns Estados não destinam nenhuma carga horária prática para sua realização. Conclusão: Apesar de os cursos apresentarem similaridade em seu perfil de oferta para a maioria dos aspectos avaliados, faz-se necessário entender se as diferenças encontradas impactam no processo de ensino-aprendizagem da disciplina estudada. Palavras-chave: Currículo. Ensino Superior. Nutrição. Educação Física.

Previous studies have shown that pinealectomy induces insulin resistance in rats. The present study aimed to verify whether pinealectomy modulates the expression of apoptosis‐related genes in rat subcutaneous adipose tissue (TA‐Sc). For this purpose, samples from the bank of frozen tissue of animals (Ethics Committee/Institute of Biomedical Sciences (ICB) Protocol No. 896/2017), belonging to the Laboratory of Adipose Tissue Physiology, University of Sao Paulo (USP), were used. The material came from Wistar rats (male and adults), whose were kept at the Experimentation Hall of the Department of Physiology and Biophysics of the ICB/USP and handled according to a certificate approved by the Ethics Committee on the Use of Animals (CEUA)/(03/10/2011, protocol no. 129) and standards established by the National Council for Control of Animal (CONCEA). The rats were housed in polypropylene boxes under controlled environmental conditions (25 ± 2 ºC) and light (12 h light/12 h dark), with water and standard ration for Nuvilab® rodents (Nuvital S/A, Colombo, PR) ad libitum. The animals, when completed 10 weeks old, were anesthetized and submitted to pinealectomy (P) by the method of Hoffman and Reiter (1965) or to the fictitious operation (C) and, following their recovery, they were kept in individual boxes. After four weeks of the surgery (14 weeks old) the animals P and C were killed by decapitation and inguinal TA‐Sc dissected. For the evaluation of gene expression related to apoptosis, samples of the Sc fat pad were submitted to the Total RNA Extraction stages; Synthesis of cDNA by Reverse Transcription and Polymerase Chain Reaction from cDNA (Real Time PCR). PCR reactions were performed by the StepOnePlus® Real Time PCR System (Applied Biosystems). The evaluated genes were: Caspases (3, 8, 9), Bax, Fas and BCL‐2. The data were analyzed according to the requirements of homoscedasticity and normality by means of the Shapiro‐Wilk test and later the Student's t‐test, unpaired and parametric. The 95% confidence interval and p value <0.05 were considered significant. The statistical program used was Graph Pad Prism version 6.0. The results showed that pinealectomy did not alter the expression of the anti and pro‐apoptotic genes evaluated (p> 0.05), with the exception of Caspase 9, whose gene expression was significantly reduced in group P in relation to C (Figure 1). In conclusion, pinealectomy is capable of selectively interfering with the gene expression of the apoptotic pathway, specifically in the caspase activator 9.Support or Funding InformationConselho Nacional de Desenvolvimento Científico e Tecnológico‐CNPqThis abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.

In adipose tissue, the expression of hundreds of genes exhibits circadian oscillation, which may or may not be affected by circulating melatonin levels. Using control and pinealectomized rats, we investigated the daily expression profile of Actb, Hprt-1, B2m, and Rpl37a, genes that are commonly used as reference genes for reverse transcription quantitative polymerase chain reaction (RT-qPCR), in epididymal (EP), retroperitoneal (RP), and subcutaneous (SC) adipose tissues. In control rats, Actb expression presented a daily oscillation in all adipose tissues investigated, Hprt-1 showed 24-h fluctuations in only RP and SC depots, B2m was stable over 24 h for EP and RP but oscillated over 24 h in SC adipose tissue, and Rpl37a presented a daily oscillation in only RP fat. In the absence of melatonin, the rhythmicity of Actb in all adipose depots was abolished, the daily rhythmicity of Hprt-1 and B2m was disrupted in SC fat, the peak expression of Rpl37a and Hprt-1 was delayed, and the amplitude of Rpl37a was reduced in RP adipose tissue. Collectively, our results demonstrate that the expression of putative reference genes displays a daily rhythm influenced by melatonin levels in a manner specific to the adipose depot. Thus, the proper standardization and daily profile expression of reference genes should be performed carefully in temporal studies using RT-qPCR analysis.