The study was made about parameters of fluctuating asymmetry of the birch leaf blade and amount of total soluble protein of the seed progeny of Betula pendula in areas of Central Black Earth Region in different environmental conditions. The environmental impact on trees of weeping birch and its seed progeny in areas with different ecological pressure was shown. The high level of fluctuating asymmetry was revealed in maternal samples growing in conditions of anthropogenic pressure. The seed progeny of Betula pendula has the increased amount of total protein in polluted areas as compared to the control group (maternal trees and its seed progeny collected in the ecologically clean territory).