Conventional
delivery systems for hydrophilic material still face
critical challenges toward practical applications, including poor
retention abilities, lack of stimulus responsiveness, and low bioavailability.
Here, we propose a robust encapsulation strategy for hydrophilic cargo
to produce a wide class of aqueous core–shell–shell
coconut-like nanostructures featuring excellent stability and multifunctionality.
The numerous active groups (−SH, −NH2, and
−COOH) of the protein–polysaccharide wall material enable
the formation of shell-cross-linked nanocapsules enclosing a liquid
water droplet during acoustic cavitation. A subsequent pH switch can
trigger the generation of an additional shell through the direct deposition
of non-cross-linked protein back onto the cross-linked surface. Using
anthocyanin as a model hydrophilic bioactive, these nanocapsules show
high encapsulation efficiency, loading content, tolerance to environmental
stresses, biocompatibility, and high cellular uptake. Moreover, the
composite double shells driven by both covalent bonding and electrostatics
provide the nanocapsules with pH/redox dual stimuli-responsive behavior.
Our approach is also feasible for any shell material that can be cross-linked via ultrasonication, offering the potential to encapsulate
diverse hydrophilic functional components, including bioactive molecules,
nanocomplexes, and water-dispersible inorganic nanomaterials. Further
development of this strategy should hold promise for designing versatile
nanoengineered core–shell–shell nanoplatforms for various
applications, such as the oral absorption of hydrophilic drugs/nutraceuticals
and the smart delivery of therapeutics.
Organotypic micrometre-size 3D aggregates of skin cells (multicellular spheroids) have emerged as a promising in vitro model that can be utilized as an alternative of animal models to test active...
Biofouling of PVAc and PVOH surfaces by fungal conidia can result in surface discolouration and subsequent biodeterioration. In order to understand the interactions of fungal conidia on polymer surfaces, the surface properties of PVAc and PVOH and the hydrophobicity, size and shape of three type of fungal conidia was determined (Aspergillus niger 1957, Aspergillus niger 1988 and Aureobasidium pullulans). Fungal conidia were used in a range of binding assays (attachment, adhesion and retention). The PVAc and PVOH demonstrated different surface topographies and the PVAc demonstrated a higher maximum height (300.6 nm) when compared to the PVOH (434.2 nm). The PVAc surfaces was less wettable (75°) than the PVOH surface (62°). The FTIR demonstrated differences in the chemistries of the two surfaces, whereby the PVOH confirmed the presence of polar moieties. Hydrophobicity assays demonstrated that both A. niger species' were more non-wettable than the A. pullulans. Following the attachment assays, the more hydrophobic Aspergillus spp. conidia attached in greater numbers to the more wettable surface and the A. pullulans was retained in greater numbers to the less wettable PVAc surface. The adhesion and retention assays demonstrated that the more polar surface retained all the types of conidia, regardless of their surface hydrophobicities. This study demonstrated that conidial binding to the surfaces were influenced by the chemistry and physicochemistry of the surfaces and spores. However, the inclusion of a washing stage influenced the adhesion of conidia to surfaces. In environments that were indicative of a attachment or retention assay a PVAc surface would reduce the number of A. niger spp. spores whilst a PVOH surface would reduce the number of A. pullulans spores. However, in an environment similar to a adhesion assay, a PVAc surface would be most beneficial to reduce spore retention. Thus, the use of the correct methodology that reflects the environment in which the surface is to be used is important in order to accurately inform hygienic surface development.
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