The research was undertaken to study the effect of frozen semen distance from liquid nitrogen surface during handling on spermatozoa quality of PE goat. Thirty straws of PE goat frozen semen was allotted into six treatments i.e. T0 (control, straws submerged in liquid nitrogen), T1 to T5 based on distance of straws to liquid nitrogen surface were 5, 10, 15, 20, and 25 cm. Variables measured were motility, viability, and abnormality of spermatozoas. The results showed that PE goat frozen semen exposed in different distance to liquid nitrogen surface decreased (P<0.05) motily and viability of spermatozoas and had no effect (P>0.05) on abnormality of spermatozoas. The motily and viability was decreased in T4 and T5. In conlusion, exposing PE goat frozen semen from liquid nitrogen surface at 15 cm or less for 5 min maintain the quality of spermatozoas.
Antibodi imunoglobulin G (IgG) merupakan salah satu immunoglobulin yang dikandung oleh antibodi anti-sperma (ASA) yang terdapat pada saluran reproduksi betina. Imunoglobulin G dapt berikatan dengan protein-protein yang berpotensi sebagai protein antigenik seperti alfa enolase (ENO1). Penelitian ini bertujuan untuk mengidentifikasi dinamika dan potensi ENO1 membran plasma spermatozoa sebagai protein antigenik serta kualitas spermatozoa sapi Bali dengan perlakuan 0,5 mg/ml IgG. Sampel penelitian adalah 16 ejakulat yang diperoleh dari 4 ekor pejantan. Motilitas spermatozoa dievaluasi menggunakan CASA, viabilitas melalui metode pewarnaan diferensial, Keutuhan Membran Plasma (MPU) menggunakan metode Hypo-osmotic Swelling Test (HOS-Test), nilai Mix Anti-globulin Reaction (MAR) diperoleh dari MAR-Test, sedangkan kuantitas ENO1 diukur dengan ELISA. Analisis data menggunakan analisis ragam RAK. Hasil penelitian menunjukkan bahwa perlakuan dengan IgG sampai 0,5 mg/ml menurunkan motilitas, kinematika spermatozoa, viabilitas, MPU dan kuantitas ENO1 membran plasma spermatozoa (P<0,01), serta meningkatkan nilai MAR-test (P<0,05). Penelitian menyimpulkan bahwa IgG dapat menurunkan kualitas spermatozoa dan kuantitas ENO1, sekaligus menunjukkan bahwa ENO1 membran plasma spermatozoa sapi Bali berpotensi sebagai protein antigenik.
Penelitian ini bertujuan untuk mengetahui pengaruh lama penyimpanan semen beku dalam es terhadap motilitas spermatozoa kerbau lumpur. Rancangan percobaan yang digunakan yaitu Rancangan Acak Lengkap dengan 5 perlakuan dan 5 kali ulangan. Perlakuan yang dilakukan meliputi: semen beku yang dithawing langsung dari nitrogen air (P0), penyimpanan dalam es selama 15 menit (P1), penyimpanan dalam es selama 30 menit (P2), penyimpanan dalam es selama 45 menit (P3), penyimpanan dalam es selama 60 menit (P4).. Peubah yang diamati adalah motilitas spermatozoa dan recovery rate. Data yang diperoleh dianalisis menggunakan sidik ragam. Hasil penelitian menunjukkan bahwa penyimpanan semen beku dalam es mempengaruhi motilitas dan recovery rate spermatozoa kerbau lumpur (P<0,05). Motilitas, dan recovery rate P0 lebih tinggi dibandingkan P1, P2, P3, dan P4 (P<0,05). Motilitas P0, P1, dan P2 masih diatas 40%. Dapat disimpulkan bahwa penyimpanan semen beku dalam es menurunkan kualitas spermatozoa kerbau, semen beku kerbau dapat disimpan selama 30 menit dalam es sebelum dithawing.
The purpose of this study was to determine the effect of various thawing temperatures to the sperm quality of FH dairy cow frozen semen. The experimental design used was completely randomized design (CRD) with 5 treatments and 5 replications . The thawing treatments were 30 seconds at 33 0C (P1), 35 0C (P2), 37 0C (P3), 39 0C (P4), 41 0C (P5) . Observed variables were sperm motility, viability and recovery rate . Data were analyzed using analysis of variance . The results showed that different thawing temperatures affect the motility , viability, recovery rate of frozen spermatozoa (P<0.05). Motility and recovery rate of P4 was higher (P< 0.05) than P2 , P1 and P5 , but did not differ (P>0.05) with P3 . Viability of P4 was higher (P>0.05) than P1 and P5 but did not differ (P>0.05) with P2 and P3. In conclusion , thawing at 39 0c and 37 0C for 30 seconds had best semen quality of FH frozen semen.
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