The Mediterranean fruit fly, Ceratitis capitata (Weidemann), is one of the most economically important tephritid species worldwide. It has spread across six geographic regions as a result of successful invasions and continues to cause substantial losses to agricultural communities. Our study examined 1,864 flies originating from 150 localities, using mitochondrial DNA sequencing methods. We tested for population structure and revealed the genetic diversity for 1,592 specimens gathered from 144 wild fly collections from 46 countries representing the entire geographic range for this species. We also include in this study 272 Sterile Insect Technique (SIT) specimens from four SIT facilities. We recovered 202 haplotypes from the current sampling and updated previously published work to reveal a total of 231 haplotypes for this pest. These data show population structure at and below the regional level for these collections, shedding light on the current demographics for this species. We observed four common haplotypes, seen among 62% of the samples sequenced that have worldwide distribution. Three haplotypes were seen in SIT flies, with one seen as the predominant haplotype. Our work showed that two of the haplotypes were private to SIT flies, not present among wild fly collections. However, a third haplotype common among wild fly collections was also seen in one SIT facility but at a low frequency based on the current sampling. We provide guidance on the interpretation of these methods for the source estimation of current and future infestations.

The Mediterranean fruit fly, Ceratitis capitata (Weidemann), is considered one of the most significant tephritid pest species worldwide and is an exotic species in most of its range. Here, we investigated polymorphism at 14 microsatellite loci for a total of 126 populations of C. capitata from six geographical regions, applying network theory and cluster analyses. Analyses revealed nine distinct modules for the Central American region and one in each of the remaining five regions. Bayesian cluster analysis revealed that the highest level of genetic partitioning corresponds with the presence of 3 well-defined genetic clusters. Our results confirm the African origin for Mediterranean populations based on genetic diversity and suggest a direct invasion of C. capitata from the Mediterranean to Central-America. South American populations show links with Central-America, but also exhibit indications of direct admixture with the European cluster. Additionally, the network analysis proposes a South American origin for the Madeiran and Hawaiian flies. Cluster analysis corroborates the hypothesis of a Mediterranean origin for Australian samples. Our work provides novel insights regarding the migration history of Medfly worldwide.

BioOne Complete (complete.BioOne.org) is a full-text database of 200 subscribed and open-access titles in the biological, ecological, and environmental sciences published by nonprofit societies, associations, museums, institutions, and presses.