Analysis of the ivater space and lipid concentration in human subcutaneous adipose tissueTotal water, extracellular and intracellular water and lipids were measured in samples of human subcutaneous adipose tissue from 22 persons of normal weight and 20 obese persons. Total water was determinded as the H 2 O space, and the extracellular water was measured as the sorbitol-[U-14 C] space. Sources o£ error in the analysis and the reproducibility of the values were tested. In the persons of normal weight, the average intracellular water was 53.9 ml/kg adipose tissue and for obese persons 52.2 ml/kg. The average extracellular water was 139.2 ml/kg and 144.0 ml/kg in two normal weight collectives, and 142.3 and 143.9 nil/kg adipose tissue in two obese collectives. During a two hour incubation period, the total water and the extracellular space increased continually. The addition of insulin or glucose to the medium did not affect the values for the water spaces. The lipid content of adipose tissue from obese persons was significantly higher.

The effect of a prolonged (3 hours) defined coronary flow restriction on early (30 minutes) and late (24 hours) reperfusability and survival of the myocardium was studied in a closed-chest pig model. Coronary blood flow (CBF) was restricted to 51 +/- 4% (moderate flow restriction) and 36 +/- 6% (severe flow restriction) of preexisting resting flow values. Regional determination of the restricted CBF after severe flow restriction showed the anticipated extension of the ischemic area from endocardial to epicardial layers and to the lateral border zone. Upon early reperfusion a hyperemic effect was observed, which reflected the preceding degree of underperfusion. The maximal hyperemic effect was found in samples with CBF restriction to 38% of the control flow values. Twenty-four hours after blood flow restitution the hyperemic effect had disappeared. At this time control flow values had not returned, where previous CBF restriction had exceeded 50%. The amount of infarcted tissue in the area supplied by the left circumflex artery was 5.7% after moderate, and 31.6% after severe flow restriction. Morphologically the infarcted myocardium consisted of disseminated necrosis after moderate, and of confluent necrosis after severe flow restriction. At flow restriction exceeding 50%, the chances of reestablishing perfusion and thus salvaging the myocardium appear minimal.

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