Otitis externa is a common complaint in dogs. Bacteria and yeasts are commonly involved and may perpetuate inflammatory reactions inside the ear canal. Otoscopy, cytological examination of secretion and microbiological culture embody forms of diagnosis. Cytology also has great use in accessing treatment evolution. Therapy usually consists of cleaning ear canals and subsequent use of antibiotics or antifungal products. As some of them may cause hypersensitivity and even ototoxicity, searching for new pharmacological bases is currently necessary and justifies this study, which aimed to evaluate in vitro and in vivo efficacy of tea tree essential oil for bacterial and yeast ear infections in dogs. Twenty-eight dogs from a particular shelter in Cuiabá (Mato Grosso, Brazil), presenting clinical signs of otitis externa, were enrolled in this clinical trial. In all of them, clinical and cytological evaluations, as well as culture and susceptibility testing of the affected ears were carried out. From each dog, one ear was treated with 5% tea tree essential oil lotion and the other with standard otic formulation, according to the type of infection (bacterial, yeast or both). In vitro susceptibility testings of all ear cultures, to the same drugs used in treatment, were also carried out. Culture results showed 62.5% bacterial and fungal infection, 33.9% bacterial infection and 3.6% fungal infection, from the 56 ear samples collected. The most common microorganisms isolated were Staphylococcus intermedius, Staphylococcus aureus, Proteus mirabilis and Malassezia pachydermatis. Gram-positive bacteria were susceptible to gentamycin in 60.5% and resistant in 16.3% of the samples. Five percent tea tree essential oil formulation produced a 5mm clear zone of inhibition around the disks in one of the 63 samples evaluated. Pure (100%) tea tree essential oil formulation produced a 10mm clear zone of inhibition around the disks in four of the 63 samples evaluated, a 9mm zone in three samples, an 8mm zone in 16 samples, a 7mm zone in seven samples, a 6mm zone in two samples and there was no clear zone in 31 samples. Inhibition zones were produced by strains of Staphylococcus intermedius, Staphylococcus hyicus, Corynebacterium sp., Proteus mirabilis and Enterobacter sp. tea tree essential oil ear solution significantly induced remission of clinical signs both in bacterial and yeast ear infections. It also reduced as much Malassezia pachydermatis ear infection as the nystatin solution used in this study, while gentamycin solution showed better antibacterial effect. More studies should be conducted to evaluate in vitro diffusion properties of tea tree essential oil. Good antimicrobial spectrum and the absence of adverse reactions confirm the importance of developing a tea tree formulation as an alternative therapy for ear infections in dogs.

The transfusion of blood components is common in a veterinary clinic; however, the safety of this therapeutic measure cannot always be guaranteed. Studies show a high risk of haemoparasite transmission during blood transfusion in canines. These parasites include Leishmania chagasi, Anaplasma platys, and Ehrlichia canis, which are endemic to the city of Cuiabá. This study aimed to evaluate the occurrence of L. chagasi, Trypanosoma cruzi, Babesia (canis) vogeli, A. platys, and E. canis in canine blood donor candidates, and identify possible factors associated with the infection of these agents. Sixty-six canines were evaluated using serologic and molecular tests, for the presence of the Leishmania species. While one canine sample showed a positive result for L. chagasi with indirect fluorescent antibody test, with titer of 1:40, and seven canine samples were positive using DPP, all other samples were negative when using PCR and ELISA. All canines were negative for T. cruzi when using PCR. The B. (c.) vogeli infection was identified in one canine and A. platys was identified in six canines. E. canis was identified in 17 canines, with a prevalence of 25.7%. There were no significant factors associated with the infection of the pathogens investigated. Given the observation of infection, even in the absence of clinical symptoms, emphasis must be placed on the need for the use of more sensitive and specific diagnostic methods for the screening of donor canines.

Background: Hemimelia is a rare anomaly in which one of paired bone is congenital or partially absent. The etiology may be related to genetic and/or environmental factors. Clinically is observed the angulation of the affected limb, resulting in functional limitations as angular deformity, lameness and decreased range of motion. The diagnosis is based on clinical fndings and radiographic examination that detects the complete or partial absence of the affected bone and angular deviations. We aim to report a case of a partial unilateral radial hemimelia in a feline diagnosed at the Veterinary Hospital of the Federal University of Mato Grosso (HOVET-UFMT).Case: We attended at the HOVET-UFMT a two years old, feline, female, domestic short hair, presenting as a complaint the presence of a friable mass in the oral cavity for a year and a deformity in the left forelimb since birth, showing swallowing diffculties, lameness and diffculty support. On clinical examination, we obeserved the left forelimb flexed and deviation inthe medial direction, shortening of the limb, no crepitation during handling and evident lameness. Radiographic examination in the craniocaudal and mediolateral projections of the thoracic limbs revealed the partial absence of the radius with just a trace of the proximal radial epiphysis, shortening and a marked ulnar curvature, producing a signifcant deviation of the limb in the cranial direction. To perform incisional biopsy of the mass in the oral cavity, the patient underwent general anesthesia, but died after the procedure. The animal was sent to the Veterinary Pathology department to perform the necropsy that confrmed the diagnosis of Gingivitis-Stomatitis-Pharyngitis Complex, evidencing a hyperplasia of the gingival epithelium and tongue, diffuse and severe lymphoplasmacytic inflammation of the lamina propria, multifocal hydropic degeneration of the basal layer with presence of necrotic acanthocytes and even multiple areas of ulceration. After routine necropsy, the dissection of the left forelimb was carried out in order to be observed macroscopically the anomaly and its presentation, confrming the radiographic fndings.Discussion: In this case, we could not determine the origin of the hemimelia because both environmental and genetic factors can influence the embryonic development of the limb, particularly in the period between the 16th and the 28th day after fertilization. The radial hemimelia is a serious congenital pathology and when presented bilaterally is often associated with other malformations. In this case, the malformation was presented in a partial way (the absence of a segment) only in the left forelimb. Macroscopically there were not any other abnormalities. The conservative treatment was instituted with the maintenance of the animal in smooth and soft surface to avoid the occurrence of ulcerations on the contact areas with the ground. It is concluded that the history and physical examination associated with radiological fndings were able to establish the defnitive diagnosis of the partial unilateral hemimelia of the left radius.Keywords: anomaly, absence, cat, limb.

Brucellosis is a chronic contagious infectious zoonosis that affects the reproductive system of animals, causing economic and health losses. This study diagnosed Brucella spp. in commercial kennels, comparing PCR positivity in different biological samples (blood, semen, and vaginal secretion), as well as correlating these findings with reproductive indices. Hence, we analyzed dogs from kennels in the neighboring cities of Cuiabá and Várzea Grande/MT, Brazil. The reproductive histories of the animals were obtained and blood samples were collected from all animals (n=35); in addition, semen samples were collected from males (n=9) and vaginal swabs were collected from females (n=24) to perform polymerase chain reactions (PCR) for brucellosis. The findings indicated that vaginal swab PCR is an effective test to identify Brucella spp. For males, there were more positive results when testing blood samples, possibly because the male animals were at the beginning stage of infection.

Background: The uterine horn agenesis or aplasia is a rare anomaly consisting of absence or incomplete development of the paramesonephric ducts that origin the uterine horns. This change occurs during fetal formation and may occur concomitantly to agenesis of the ovary and/or ipsilateral kidney. The extra-uterine pregnancy consists of fetal growth outside the uterine cavity and can occur as a tubal or abdominal pregnancy. We report here the case of a 7-years-old mix breed dog, attended at the Veterinary Hospital of the Federal University of Mato Grosso, with accidental diagnosis of agenesis of uterine horn and ovaries and ectopic fetal mummifcation.Case: A 7-years-old mix breed dog, not spayed, was attended at the Veterinary Hospital of the Federal University of Mato Grosso (HOVET-UFMT), complaining of mucous bloody vaginal discharge. The patient had regular estrus and was multiparous. There was no mating history in the last estrus neither trauma over the past months. In previous pregnancies the patientpresented eutocic labors. On physical examination the dog showed apathy although it was alert, pale mucous membranes, presence of perivulvar blackish secretion and discreet abdominal enlargement. As preoperative exams, a complete blood count, liver and renal function (serum creatinine and alanine aminotransferase) were performed. It has also performedthe abdominal ultrasound examination, which revealed the presence of uterus flled with hypoechoic luminal content and a thickened and irregular wall. The patient was referred to therapeutic ovariohysterectomy (OHE). After anesthesia and antisepsis, the access to the abdominal cavity was made, where there was a mummifed fetus with approximately 15.5 cmfrom neck to tail set, equivalent to 57 days of gestation. After removal of the papyraceus fetus, the uterus was found and then, when exposed, there was only the left uterine horn flled by content and ovarie, both were removed. After inspection, the synthesis of the abdominal cavity was carried out as the routine. During the postoperative period, the patient had no complications or other clinical signs similar to those observed in the initial presentation. Antibiotic (enrofloxacin 5 mg/kg every 12h), analgesic (dipyrone 20 mg/kg every 8 h) and non-steroidal anti-inflammatory (meloxicam 0.2 mg/kg every 24 h) were prescribed. The fetus and uterus were sent to the Veterinary Pathology UFMT Laboratory (PVL-HOVET).The fetus was covered by omentum and fat. After the initial incision, there was a hard fbrous capsule surrounding the mummifed fetus. There were no recent signs of rupture or scarring resulting from previous trauma in the uterus. Its size was 15x4.8x2.8 cm and it was flled out with mucus bloody brownish secretion (pyometra). Microscopically, had purulent inflammatory infltrate, diffuse and severe in mucosa and submucosa associated with cell debris and moderate hyperplasia glands containing microabscesses inside.Discussion: Reports of extra-uterine fetal mummifcation in dogs and cats are scarce. Even being reported infrequently in dogs if congenital uterine and ovarian abnormalities are found during surgery, a detailed inspection of the abdominal cavity should be performed in order to rule out the possible presence of the ipsilateral ovary. Even with the lack of specifc clinical signs, fetal ectopy can be diagnosed through a detailed clinical examination and image exams. Exploratory laparotomy can be used as a diagnostic and therapeutic tool, since the treatment is the excision of ectopic tissue and OHE in cases of uterine rupture.Keywords: aplasia, fetus, mummifcation, unicornuate uterus.