No abstract

Purpose.The epidemiology of melioidosis in Vietnam, a disease caused by the soil bacterium Burkholderia pseudomallei, remains unclear. This study aimed to detect pediatric melioidosis in South Vietnam and describe clinical features and the geographical distribution. Methods. We introduced a simple laboratory algorithm for detecting B. pseudomallei from clinical samples at Children’s Hospital 2 in Ho Chi Minh City in July 2015. B. pseudomallei culture isolates were confirmed by molecular methods. A retrospective observational study of children aged < 16 years with culture-confirmed melioidosis between July 2015 and August 2019 was undertaken. Results.Thirty-five pediatric cases of melioidosis were detected, with cases originating from 13 out of 32 provinces and cities in South Vietnam. The number of pediatric melioidosis cases detected from a certain region correlated with the overall number of inpatients originating from the respective geographical area. Suppurative parotitis (n = 15; 42.8 %) was the most common clinical presentation, followed by lung infection (n = 10; 28.6 %) and septicemia (n = 7; 20 %). Fourteen (40 %) children had disseminated disease, including all cases of lung infection. Four (11.4 %) deaths occurred in the disseminated disease group. Conclusions. We report here the first series of pediatric cases of melioidosis from Vietnam detected in a single big referral children’s hospital in Ho Chi Minh City. The patients’ origin indicates a wide distribution of melioidosis in South Vietnam. It seems probably that cases not only in children but also in adults remain grossly undiagnosed. Further awareness raising and laboratory capacity strengthening are needed in this part of the country.

No abstract

The supramolecular complex formation in aqueous solution between fatty acids (with the content of αeleostearic acid more than 70%) and β-cyclodextrin, βCD, was supported by FT-IR and UV spectroscopies as well as by XRDanalysis. RP HPLC was utilized for investigation of the content of fatty acid in the inclusion complex and their stability. All results showed that the molecules of fatty acids were successfully included in the β-cyclodextrin cavity. The yield of the inclusion complex is up to 30.1% with a fatty acid content of 20.5%. Due to the formation of inclusion complex, the solubility, and stability of conjugated acids significant increase.

Поступила в редакцию 3 июля 2018 г, после доработки -14 сентября 2018 г. С использованием обращенно-фазовой высокоэффективной жидкостной хроматографии с диодно-матричным и масс-спектрометрическим детектированием исследован видовой состав антоцианов цветков Catharanthus roseum. После кислотного гидролиза антоцианов по характерному изменению электронных спектров поглощения и по изменению удерживания было установлено, что все пять антоцианидинов цветков фиолетового цвета отличаются от пяти наиболее часто встречающихся в природе (в виде гликозидов) антоцианидинов однотипным дополнительным метилированием. Направление метилирования по гидроксильной группе в положении 7 флавилиевого скелета было определено при анализе изменения параметров электронных спектров поглощения; таким образом, антоцианы цветков катарантуса построены на пяти необычных антоцианидинах,являясь производными 7-метилдельфинидина, 7-метилцианидина, 7-метилпетунидина, 7 метилпеонидина (розинидина) и 7 метилмальвидина (хирсутидина). Сопоставление масс-спектрометрических данных, электронных спектров поглощения и параметров удерживания антоцианов цветков Catharanthus roseum указывают на присутствие в экстрактах лепестках цветков 3-рамозилгалактозидов указанных антоцианидинов, к которым добавляются (в меньшем количестве) 3-галактозиды.Using the reversed phase high performance liquid chromatography with diode-array and massspectrometric (in ESI mode) detection, a composition of Catharanthus roseum petals was established. After the acid hydrolysis, all five anthocyanidins were found to be different comparing to the ordinary anthocyanidins from Vitis vinifera fruits. The anthocyanins were elucidated to be 7-O-methyl derivatives of delphinidin, cyanidin, petunidin, peonidin and malvidin by the analysis of retention in RP HPLC, mass-and UV-visible spectra. The anthocyanins were characterized with UV-visible spectra, having the same fixtures as the set of nonmethylated (in position 7) anthocyanin with hypsochromic (4 nm) of spectral maxima. The absorption bands for 7-methylcyanidin and 7-methylpeonidin aglycons and derivatives were indistinguishable while for the set of 7-methydelphinidin, 7-methypetunidin and 7-methylmalvidine (hirsutidin) a consecutive shift of absorption maxima by approximately 1 -1.5 nm was found. The same was true for non-methylates at position 7 derivatives. The analysis of retention of anthocyanins of the flowers including the comparison with the retention of Mangifera indica skin anthocyanins, mass-and UV-visible spectra indicated that a minor set of anthocyanins included two