Mosquitoes in general and Culex quinquefasciatus in particular have for a long time constituted a source of nuisance due to the diseases they transmit, stings and annoying buzzing. They are also a public health problem. This is why this study is aimed at finding a biopesticide that can fight effectively but also be an alternative to chemical residual pesticides in the environment. Concentrations of biopesticides used are ranged from 2.5 10 8 to 12.5 10 8 spores/ml with intervals of 2.5 10 8 spores/ml for Metarhizium anisopliae. Concentrations ranging beetween 8 10-4 g/ml to 40 10-4 g/ml with intervals of 8 10-4 g/ml of Azadirachta indica (Suneem 1%) were used to fight against 100 three instars larvae of Culex quinquefasciatus. Concentrations of the mixture of Azadirachta indica (Sunnem 1%) and Metharhium anisopliae ranging from 0.02 ml +2 10 8 spores/ml to 0.02 ml + 1 10 8 spores/ml were used to study synergic effects of these two biopesticides. One hundred (100) of three instars larvae of Culex quinquefasciatus are used as control and placed in the same jars with 500 ml of distilled water. Metarhizium anisopliae (green muscle) have caused 50% mortality of three instars larvae of Culex quinquefasciatus after 3 days. Azadirachia indica (Suneem 1%) have gaved a mortality more than 60% after 2 days. The mixture Azadirachta indica (Suneem1%) and Metarhizuim anisopliae caused a high mortality with a rate that exceeds 90% in 3 days. The mixture also prevented a larval moult thus stopping their growth and development. In summary, both Metharizium anisopliae and Azadirachta indica (Suneem 1%) are effective against three instars larvae of Culex quinquefasciatus. Their mixture caused a synergic effect and thus increased their individual efficacy of two biopesticides.
Morphometric data is a useful complement of information that allows precise identification of the different parts of Rastrococcus invadens in Senegal. This study was carried out in four farms (Diatock and Oussouye) all located in natural Casamance and (Santhie and Khay) in the region of Thies. The work took place in February 2016 where citrus production is important. In each farm, we chose the lemon tree and the orange tree which are host plants of R. invadens.From each plant we collected 10 specimens. This enabled us to get 40 specimens from Casamance and 40 other specimens from Thies. Specimens were coded with regard to both the area and the type of plant they were collected from. The average measurements of the different parts of the insect showed a difference between the populations of the two agro-ecological zones with homogeneity of the populations of Thies and a heterogeneity of the populations of Casamance with a large zone of introgression. The results of this study should allow easier recognition of the different parts of R. invadens.
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