1 We investigated the effects of rotigaptide (ZP123), a stable hexapeptide with antiarrhythmic properties, on gap junction mediated intercellular communication in contracting rat neonatal cardiac myocytes, HL-1 cells derived from cardiac atrium and in HeLa cells transfected with cDNA encoding Cx43-GFP, Cx32-GFP, Cx26-GFP, wild-type Cx43 or wild-type Cx26. 2 Intercellular communication was monitored before and after treatment with rotigaptide following microinjection of small fluorescent dyes (MWo1 kDa). The communication-modifying effect of rotigaptide was confined to cells expressing Cx43 since the peptide had no effect on dye transfer in HeLa cells expressing Cx32-GFP, Cx26-GFP or wild-type Cx26. In contrast, HeLa cells expressing Cx43-GFP exposed to 50 nM rotigaptide for 5 h showed a 40% increase in gap junction mediated communication.3 Rotigaptide (50 nM) increased intercellular dye transfer in myocytes and atrial HL-1 cells, where Cx43 is the dominant connexin. However, it caused no change in cell beating rates of cardiac myocytes. 4 Western blot analysis showed that rotigaptide did not modify the overall level of Cx43 expression and changes in the phosphorylation status of the protein were not observed. 5 We conclude that the effects of rotigaptide were confined to cells expressing Cx43.

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The increased penetration of renewable energy resources poses challenges for grid stability. The stochastic generation of solar and wind power cannot be controlled to follow load. And, the transition away from synchronous generators is reducing the capacity to arrest and recover from frequency disturbances. Smart electric water heaters provide utilities with an appliance that can be remotely controlled and serve as a form of energy storage. They have very fast response times and make up a large amount of residential energy consumption, making them useful for load peak shifting as well as other ancillary grid services. As smart appliances become increasingly widespread, more and more devices can be brought into the utility control network and aggregated into a flexible resource on a multi-megawatt scale. This paper demonstrates the usefulness of aggregated electric water heaters for providing two ancillary services: peak shifting and frequency response. Because a large number of assets are required, emulators are developed based on observations of real devices. Emulated water heaters are then connected to an energy resource aggregator using an internet-of-things network. The aggregator uses these assets to shift consumption away from peak hours and for detecting upward frequency disturbances.

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