It is of the greatest importance in tests of the sanitary quality of water and milk supplies that organisms which specifically cause infectious diseases through such sources shall be subject to methods of rapid and definite isolation. Up to the present time this has been a matter of great difficulty and uncertainty owing to the fact that the disease germs which occur in water and especially in milk are intermingled with, and greatly outnumbered by, other intestinal germs and many species of common so-called air and water bacteria. CHEMICAL PRECIPITATION AND AGGLUTINATION. Many methods have been employed in isolating or attempting to isolate the typhoid bacillus from water. Processes involving chemical precipitation have been studied by Vallet,' Schuder," Ficker,' Mtiller,s Willson," Nietor,? and Ditthorn and Gildemeister,? the latter using ox bile as an enrichment medium after chemical precipitation. Agglutination has also been used in enrichment methods for the isolation of B. typhosus by Adami and Chopin," Klotz." Shipilewski,xo and Altschuler. II INDICATOR DYES AND DIFFERENTIAL CHEMICALS.

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The treatment with solvent lasted for 18 hours, and a motor-driven shaking device produced thorough mixture of sample and solvent. The hot extractions were made with boiling solvent in a flask fitted with a reflux condenser. Table III SolventTrin.Ber.

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