The gene encoding the porcine growth hormone (GH) has been localized to the q-arm of chromosome 1 2 using high-resolution R-banded chromosomes for in situ hybridization. We report here the localization of GH on the p-arm of this chromosome when using in situ hybridization on high-resolution G-banded chromosomes. Sequential Q-and R-banding show that this discrepancy is caused by a reversed orientation of chromosome 12 in the R-banded high-resolution karyotype published by Rsnne et al. (1987) and the G-banded standard karyotype.

Restriction fragment length polymorphisms (RFLPs) were described for the porcine loci for 0-glucosidase (GBA) and the p-polypeptide 1 of the Na+,K+-transporting ATPase (ATPlBl). Linkage analyses using a three-generation pedigree provided evidence for the assignment of ATPlBl, GBA and two microsatellite loci (SO001 and S0067) to a previously described linkage group comprising the loci for blood group L (EAL) and an anonymous microsatellite (s0097). The linear order of the six markers was determined with confidence by multipoint analyses and the length of the linkage group was estimated at 88 CM. This linkage group was assigned to pig chromosome 4 on the basis of a previous physical localization of the ATPlBl gene. In situ hybridization data for SO001 presented in this study were consistent with a localization on chromosome 4 and suggested a regional localization to 4p12-pl3. The present study reveals conflicting data concerning the genetic localization of the K88 loci controlling the expression of the receptors for the E. coli pilus antigens. One group has reported data suggesting a loose linkage between K88 and EAL, now mapped to chromosome 4, whereas two other groups have found linkage between K88 and the transferrin locus (TF), mapped to chromosome 13 by in situ hybridization.

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