Preset larval Mercenaria mercenaria were exposed to nominal concentrations of 1 (control) to 495 microg Cu/L in artificial seawater and monitored for mortality, activity, development, and metamorphosis in sealed 30-mm plastic petri plates containing 1.5 ml of artificial seawater or toxicant solution. The plastic petri plates sorbed only about 2.6 microg/L at any dose and allowed direct observation of larval clams under a light microscope for a period of two weeks; control survivorship was in excess of 60% at 400 h. The dose-response curve for mortality for clams exposed to copper and fed Isochrysis galbana was characterized by survival similar to or better than controls at doses of 5 and 14 microg Cu/L, while doses of 7 and > or = 29 microg Cu/L exhibited mortality greater than controls. Values of lowest concentration at which 50% of the organisms died (LC50) were 62.4, 21.2, and 11.7 microg Cu/L, and the lowest observed adverse effect concentration values of 57, 29, and 29 microg Cu/L were determined at 48, 96, and 192 h, respectively. In contrast, activity, as judged by swimming, exhibited a typical exponentially decreasing response at these same concentrations. Experiments on the uptake of dissolved copper by I. galbana confirmed literature reports that these algae concentrate copper. Ingesting copper-containing algae was demonstrated to be a source of copper toxicity for larval clams.

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Preset larval Mercenaria mercenaria were exposed to nominal concentrations of 1 (control) to 495 microg Cu/L in artificial seawater and monitored for mortality, activity, development, and metamorphosis in sealed 30-mm plastic petri plates containing 1.5 ml of artificial seawater or toxicant solution. The plastic petri plates sorbed only about 2.6 microg/L at any dose and allowed direct observation of larval clams under a light microscope for a period of two weeks; control survivorship was in excess of 60% at 400 h. The dose-response curve for mortality for clams exposed to copper and fed Isochrysis galbana was characterized by survival similar to or better than controls at doses of 5 and 14 microg Cu/L, while doses of 7 and > or = 29 microg Cu/L exhibited mortality greater than controls. Values of lowest concentration at which 50% of the organisms died (LC50) were 62.4, 21.2, and 11.7 microg Cu/L, and the lowest observed adverse effect concentration values of 57, 29, and 29 microg Cu/L were determined at 48, 96, and 192 h, respectively. In contrast, activity, as judged by swimming, exhibited a typical exponentially decreasing response at these same concentrations. Experiments on the uptake of dissolved copper by I. galbana confirmed literature reports that these algae concentrate copper. Ingesting copper-containing algae was demonstrated to be a source of copper toxicity for larval clams.

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