Fruit fly (Bactrocera spp.) is one of the most important pest of crystal guava. The use of bottle as a trap with methyl eugenol block (ME block) as an attractant is commonly applied to control the population of fruit fly. The objective of this research was to determine the most effective height level of the trap to capture fruit fly. This experiment was conducted at the Great Giant Foods, Lampung. The experiment was arranged in the randomized block design which consisted of 6 treatments and 5 replications. The treatments were trap height level at 50 cm, 75 cm, 100 cm, 125 cm, 150 cm, and 175 cm above the ground. Each trap was laced with 2 ml of ME block. The results showed that height variations affected the number of captured fruit fly in crystal guava field. The best trap for catching fruit flies on crystal guavas that have been produced for more than a year old that have 150 cm tall plants is one with a height level of 125 cm. The fruit flies caught were identified as Bactrocera dorsalis and B. carambolae.
A novel biocontrol delivery system by using graphite was proven effective in controlling bacterial plant pathogens. In this study, a consortium of biocontrol agents, consisting of Bacillus subtilis and Trichoderma harzianum, was formulated in graphite added with silica nano particles (NPs). B. subtilis is an endophytic bacterium, while T. harzianum is a rhizosphere fungus. The formulation was in vitro tested on Phytophthora infestans, the cause of leaf blight on tomatoes. The objective of the experiment was to study the ability of single and consortium of B. subtilis and T. harzianum, formulated in graphite and silica NPs, in inhibiting the in vitro growth of P. infestans compared to the non-formulated ones. The experimental design was the randomized complete block design comprised of eight treatments and replicated four timens. The eight treatments were dual cultures of the consortium and the pathogen on V-8 medium as follow: B. subtilis only, B. subtilis + graphite + silica NPs, T. harzianum only, T. harzianum + graphite + silica NPs, B. subtilis + T. harzianum, B. subtilis + T. harzianum + graphite + silica NPs, graphite + silica NPs, and a control. It was concluded that the abilities of both single and consortium of B. subtilis and T. harzianum, formulated with graphite and silica NPs, in inhibiting the in vitro growth of P. infestans were the same as the nonformulated ones. This proved that the formulation in graphite added with silica NPs in the biocontrol delivery system did not reduce the ability of the biocontrol agents to control P. infestans in vitro. The pathogen growth inhibitions caused by the single and consortium of B. subtilis and T. harzianum formulated with graphite and silica NPs were ranged from 35 -54%, compared to the non-formulated ones 24.19 -49.48%.
Penelitian tentang kajian dampak kegiatan seismik 3D terhadap beberapa sifat tanah yang berdampak pada keanekaragaman hayati dan ketahanan pangan dilakukan di Desa Sambimaya, Kecamatan Juntinyuat, Kabupaten Indramayu. Kegiatan ini bekerjasama dengan para ahli dari Institut Pertanian Bogor sebagai mitra. Kajian terhadap beberapa sifat tanah akibat adanya kegiatan eksplorasi migas dengan metode 3D yang berdampak pada keanekaragaman hayati ini lebih menekankan pada jenis-jenis tumbuhan di sekitar areal studi, terutama jenis tanaman jeruk, mangga, dan padi. Hal ini karena lokasi studi merupakan lahan budidaya tanaman pertanian. Pengolahan dan analisis data dilakukan melalui pendekatan regresi untuk mengetahui hubungan dampak seismik dengan kondisi tanaman, baik tanaman jeruk, mangga, maupun padi sawah. Pengolahan data juga dilakukan menggunakan pendekatan ANOVA (analysis of variance) dan Uji t-Student untuk mengetahui faktor-faktor yang menentukan keberhasilan produksi tanaman. Tidak ada kerusakan pada tanaman padi sawah, jeruk, dan mangga yang diakibatkan oleh kegiatan seismik 3 D yang dibuktikaan dengan tidak dijumpainya tanaman padi sawah yang mengalami kerusakan atau kematian tanaman, kecuali kerusakan akibat terinjak-injaknya tanaman oleh tenaga kerja seismik. Demikian pula dengan jenis pohon jeruk dan mangga yang mengalami kerusakan atau kematian bahkan tanda- tanda kematian seperti kerontokan (gugur) daun, pengeringan ranting dan cabang setelah dua minggu pasca seismik. Kegiatan seismik 3 D tidak berpengaruh terhadap tingkat kesuburan tanah, yang dibuktikan dengan tidak adanya perubahan antara kondisi kandungan hara tanah pada pra seismik dengan pasca seismik. Kegagalan budidaya tanaman hortikultura terutama tanaman jeruk lebih diakibatkan oleh serangan hama dan penyakit yang telah ada sebelum kegiatan seismik dilakukan.
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