Lacaziosis, formerly called as lobomycosis, is a zoonotic mycosis, caused by Lacazia loboi, found in humans and dolphins, and is endemic in the countries on the Atlantic Ocean, Indian Ocean and Pacific Ocean of Japanese coast. Susceptible Cetacean species include the bottlenose dolphin (Tursiops truncatus), the Indian Ocean bottlenose dolphin (T. aduncus), and the estuarine dolphin (Sotalia guianensis); however, no cases have been recorded in other Cetacean species. We diagnosed a case of Lacaziosis in a Pacific white-sided dolphin (Lagenorhynchus obliquidens) nursing in an aquarium in Japan. The dolphin was a female estimated to be more than 14 years old at the end of June 2015 and was captured in a coast of Japan Sea in 2001. Multiple, lobose, and solid granulomatous lesions with or without ulcers appeared on her jaw, back, flipper and fluke skin, in July 2014. The granulomatous skin lesions from the present case were similar to those of our previous cases. Multiple budding and chains of round yeast cells were detected in the biopsied samples. The partial sequence of 43-kDa glycoprotein coding gene confirmed by a nested PCR and sequencing, which revealed a different genotype from both Amazonian and Japanese lacaziosis in bottlenose dolphins, and was 99 % identical to those derived from Paracoccidioides brasiliensis; a sister fungal species to L. loboi. This is the first case of lacaziosis in Pacific white-sided dolphin.

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Paracoccidioidomycosis ceti (PCM-C) is a zoonotic mycosis characterized by chronic granulomatous keloidal dermatitis in cetaceans that has been reported worldwide. The host cetacean species of PCM-C are five dolphins, namely Atlantic bottlenose dolphin (Tursiops truncatus), Indo-pacific bottlenose dolphin (T. aduncus), Pacific white-sided dolphin (Lagenorhynchus obliquidens), estuarine dolphin (Sotalia guianensis; also known

A captive male Indo-Pacific bot'tlenose dolphin (Tursiops aduncus) aged >45 years was reared in Okinawa Churaumi Aquarium (Japan). The animal had a history of a hemorrhagic ulcered mass (4 cm × 2 cm) on the right buccal mucosa that grew over a 2-year period (Figure 1) for which cytology and biopsy of the mass were performed to make a diagnosis.The oral lesion was scraped with a toothbrush to collect cells for the cytologic analysis, and the cells were immediately immersed and stirred in a large test tube containing 20 mL of CytoRich Red Preservative Fluid (Becton Dickinson). A liquid-based cytology (LBC) slide of the above sample was prepared using a modified SurePath method (Becton Dickinson) (Table 1; Figure 2). Three different SurePath slides were stained with Papanicolaou stain (Table 2), and p53 and p16 immunocytochemistry. For the p53 immunocytochemistry, antigen retrieval was performed with a citrate buffer (pH 6.0) heated at 90°C for 20 minutes. Then, a commercially available mouse, anti-human wild-type p53 protein antibody (clone DO-7,

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