Background: Linagliptin is prescribed as a dipeptidyl peptidase-4 (DPP-4) inhibitor. Azithromycin is specified as an antibiotic that binds with 23s rRNA of the 50s ribosomal subunit obstructing the microbial protein synthesis. Our study focuses on the drug-drug interactions of these drugs. Objective: The purpose of the study is to understand the bioavailability and physicochemical approaches of Linagliptin and Azithromycin interaction mediated through the strength and nature of the complexation. Methods: The assessment drug interaction applying Ultraviolet-visible spectroscopy (UV/VIS), Ultra-Performance Liquid Chromatography (UPLC), Fourier transform infrared spectroscopy (FT-IR), and Differential scanning calorimetry (DSC) for In Vitro assessment. Also, Oral Glucose Tolerance Test (OGTT) in a mice model for In Vivo. Results: The mild variation observed at different pH at a specific temperature on Job's and Ardon's equation. On UPLC, the drug mixture is 2013793 and 54631 on 50 mg/l. The height of the drug mixture 579234 and 11442, respectively. The Azithromycin, the wavelength of 731.02 cm-1, 993.34 cm-1, 1379.10 cm-1, and 1718.58 cm-1 diminish from the mixture. Also, from Linagliptin, the wavelength of 1363.67 cm-1, 1473.62 cm-1, 1718.58 cm-1 declines from the drug mixture. The melting endotherm at 125.55°C of melting normalized energy of -3.0 W/mg and 225.75°C with melting normalized energy of -5.5 W/mg of the drug mixture on DSC. In the OGTT test, the blood glucose level decrease Linagliptin and the drug mixture at (13.58 %) and (57.25%). Conclusion: Hence, the concomitant administration of Linagliptin and Azithromycin at a time might reduce the therapeutic effect of the formation of complexation.

The purpose of this study was to understand and investigate the phytochemical and antimicrobial effects of different fractions of the methanolic extract of Corchorus capsularis leaf. For the extraction of lipophilic compounds with the help of methanol solvent system, cold extraction process was used, where the grinded coarse powder of the leaf was soaked in methanol and kept for four days for proper extraction. After that, the extract was concentrated by using rotatory evaporator and dried in room temperature. Compounds in the extract were separated according to their polarity by using vacuum liquid chromatography (VLC) and solvents of different polarity N-hexane, benzene, diethyl ether, chloroform, dichloromethane. Later, thin layer chromatography (TLC), flavonoid assay, disk diffusion, inhibitory action in nutrient broth was done for different fractions of the methanolic extract. Diethyl ether, chloroform, dichloromethane fractions showed good anti-oxidant property but poor antibacterial activity against different types of microorganism. Chloroform fraction showed good inhibitory activity against Aspergilus niger (fungus) in nutrient broth.